Hu Jieqiong, Du Xijin, Huang Cui, Fu Dongjie, Ouyang Xiaobai, Wang Yake
The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, People's Republic of China; West China Dental Clinic, 36-2 Xinglong Road, Jiangbei District, Chongqing, People's Republic of China.
J Dent. 2013 Oct;41(10):927-34. doi: 10.1016/j.jdent.2013.07.014. Epub 2013 Aug 1.
To evaluate the effect of the addition of epigallocatechin-3-gallate (EGCG) on the antibacterial and physical properties of glass ionomer cement (GIC).
A conventional GIC, Fuji IX, was used as a control. EGCG was incorporated into GIC at 0.1% (w/w) and used as the experimental group. Chlorhexidine (CHX) was added into GIC at 1% (w/w) as a positive control. The anti-biofilm effect of the materials was assessed by a colorimetric technique (MTT assay) and scanning electron microscopy (SEM). The leaching antibacterial activity of the materials on Streptococcus mutans was evaluated by an agar-diffusion test. The flexural strength of the materials was evaluated using a universal testing machine and the surface microhardness was measured using a microhardness tester. The fluoride-releasing property of the materials was tested by ion chromatography.
The optical density (OD) values of the GIC-EGCG group were significantly decreased at 4h compared with the GIC group, but only a slightly decreased tendency was observed at 24h (P>0.05). No inhibition zones were detected in the GIC group during the study period. Significant differences were found between each group (P<0.05). Compared with the control group, there was a significant increase in the flexural strength and surface microhardness for the GIC-EGCG group (P<0.05). The fluoride ion release was not influenced by EGCG-incorporation (P>0.05).
These findings suggested that GIC-containing 0.1% (w/w) EGCG is a promising restorative material with improved mechanical properties and a tendency towards preferable antibacterial properties.
Modification of the glass ionomer cements with EGCG to improve the antibacterial and physical properties showed some encouraging results. This suggested that the modification of GIC with EGCG might be an effective strategy to be used in the dental clinic. However, this was only an in vitro study and clinical trials would need to verify true outcomes.
评估添加表没食子儿茶素-3-没食子酸酯（EGCG）对玻璃离子水门汀（GIC）抗菌性能和物理性能的影响。
使用传统的GIC（Fuji IX）作为对照。将EGCG以0.1%（w/w）的比例加入GIC中作为实验组。将氯己定（CHX）以1%（w/w）的比例加入GIC中作为阳性对照。通过比色技术（MTT法）和扫描电子显微镜（SEM）评估材料的抗生物膜效果。通过琼脂扩散试验评估材料对变形链球菌的浸出抗菌活性。使用万能试验机评估材料的弯曲强度，使用显微硬度计测量表面显微硬度。通过离子色谱法测试材料的氟释放性能。
与GIC组相比，GIC-EGCG组在4小时时的光密度（OD）值显著降低，但在24小时时仅观察到轻微下降趋势（P>0.05）。在研究期间，GIC组未检测到抑菌圈。各组之间存在显著差异（P<0.05）。与对照组相比，GIC-EGCG组的弯曲强度和表面显微硬度显著增加（P<0.05）。EGCG的加入对氟离子释放没有影响（P>0.05）。
这些发现表明，含有0.1%（w/w）EGCG的GIC是一种有前景的修复材料，具有改善的机械性能和较好的抗菌性能趋势。
用EGCG对玻璃离子水门汀进行改性以改善抗菌和物理性能显示出一些令人鼓舞的结果。这表明用EGCG对GIC进行改性可能是牙科临床中一种有效的策略。然而，这只是一项体外研究，需要进行临床试验来验证实际效果。
