Dentistry Biomaterials Laboratory (Biomma), School of Dentistry, Federal University of Maranhão (UFMA), 65080-805, São Luís, MA, Brazil.
University Ceuma (UNICEUMA), School of Dentistry, R. Josué Montello, 1, Renascença II, 65075-120, São Luis, MA, Brazil.
J Mech Behav Biomed Mater. 2024 Oct;158:106678. doi: 10.1016/j.jmbbm.2024.106678. Epub 2024 Jul 31.
Analyze the effects of the functionalization of pre-functionalized GIC particles with chlorhexidine on the physicochemical properties and antimicrobial activity.
Four groups were prepared: (1) GIC (Bioglass R - Biodinamica) - control group; (2) GIC-CHX 1%: Group containing 1% pre-reacted CHX particles; (3) GIC-CHX 2.5%: Group containing 2.5% pre-reacted CHX particles; (4) GIC-CHX 5%: Group containing 5% pre-reacted CHX particles. Hourglass-shaped specimens (10 mm × 2 mm x 1 mm) were fabricated for mechanical tests including cohesive strength (n = 12), modulus of elasticity (n = 12) and microhardness (n = 10). Discs (10 mm × 2 mm) were prepared for the analysis of Ca, PO and F ions release (n = 3), and roughness (n = 12). To evaluate the setting time, a Gilmore needle was used according to ISO 9917-1:2016. Disk-shaped specimens (5 × 1mm) were manufactured and subjected to bacterial activity (n = 9) (Streptococcus mutans ATCC 159).
Modulus, roughness, setting time and ions release (Ca, PO, and F) there were no statistically significant differences among the groups (p > 0.05). The setting time did not change with the incorporation of CHX. The GIC-CHX 2.5% and GIC-CHX 5% groups exhibited superior antibacterial activity compared to the control group and GIC-CHX 1% (p < 0.001). The GIC-CHX 5% group showed the highest microhardness values (p < 0.041), cohesive strength (p < 0.009) when compared to the control group.
The pre-reacted CHX in GICs was able to confer antimicrobial activity, improve cohesive strength, microhardness, and did not impair ion release, setting time, and roughness.
分析用氯己定对预官能化玻璃离子水门汀（GIC）颗粒进行功能化处理对其理化性能和抗菌活性的影响。
制备了 4 组样品：（1）GIC（Bioglass R - Biodinamica）-对照组；（2）GIC-CHX 1%：含 1%预反应氯己定颗粒的组；（3）GIC-CHX 2.5%：含 2.5%预反应氯己定颗粒的组；（4）GIC-CHX 5%：含 5%预反应氯己定颗粒的组。制备沙漏形试件进行力学性能测试，包括内聚强度（n=12）、弹性模量（n=12）和显微硬度（n=10）。制备圆盘进行 Ca、PO 和 F 离子释放（n=3）和粗糙度（n=12）分析。根据 ISO 9917-1:2016，采用 Gilmore 针评估凝固时间。制作圆盘状试件（5×1mm），进行细菌活性（n=9）（变异链球菌 ATCC 159）测试。
各组间弹性模量、粗糙度、凝固时间和离子释放（Ca、PO 和 F）均无统计学差异（p>0.05）。氯己定的加入并未改变凝固时间。与对照组和 GIC-CHX 1%组相比，GIC-CHX 2.5%和 GIC-CHX 5%组具有更好的抗菌活性（p<0.001）。与对照组相比，GIC-CHX 5%组的显微硬度值（p<0.041）、内聚强度（p<0.009）最高。
GIC 中预反应的氯己定能够赋予抗菌活性，提高内聚强度和显微硬度，且不会影响离子释放、凝固时间和粗糙度。
