CNRS, CRPP, UPR 8641, Pessac F-33600, France; University of Bordeaux, CRPP, UPR 8641, Pessac F-33600, France.
Biosens Bioelectron. 2013 Dec 15;50:478-85. doi: 10.1016/j.bios.2013.07.014. Epub 2013 Jul 16.
Bilirubin oxidases, a sub class of the Multicopper oxidases family, were discovered in 1981 by Tanaka and Murao (Murao and Tanaka, 1981) and first used for the detection of bilirubin. Since 2001 and the pioneering work of Tsujimura, these BODs have attracted a lot of attention for the reduction of O2. Unlike laccases, these BODs are stable in physiological conditions (20mM phosphate buffer, pH 7.4, 0.14 M NaCl, 37 °C) and more than 120 papers have been published in the last 7 years. Here, we will first briefly describe some general features of BODs and then review the use of BODs for bilirubin biosensors and the recent achievements and progress toward the elaboration of efficient O2 reducing cathodes.
胆红素氧化酶，多铜氧化酶家族的一个子类，于 1981 年由 Tanaka 和 Murao（Murao 和 Tanaka，1981）发现，并首次用于胆红素的检测。自 2001 年 Tsujimura 的开创性工作以来，这些 BOD 因其对 O2 的还原而引起了广泛关注。与漆酶不同，这些 BOD 在生理条件下稳定（20mM 磷酸盐缓冲液，pH7.4，0.14MNaCl，37°C），并且在过去 7 年中已经发表了 120 多篇论文。在这里，我们将首先简要描述 BOD 的一些一般特征，然后综述 BOD 在胆红素生物传感器中的应用，以及最近在阐述高效 O2 还原阴极方面的进展和成果。
