State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 61 Dai Zong Street, Tai'an, 271018 Shandong, PR China.
Plant Physiol Biochem. 2013 Oct;71:112-20. doi: 10.1016/j.plaphy.2013.07.004. Epub 2013 Jul 19.
Calcium-dependent protein kinases (CDPKs) play essential roles in calcium-mediated signal transductions in plant response to abiotic stress. Several members have been identified to be regulators for plants response to abscisic acid (ABA) signaling. Here, we isolated a subgroup I CDPK gene, ZmCPK4, from maize. Quantitative real time PCR (qRT-PCR) analysis revealed that the ZmCPK4 transcripts were induced by various stresses and signal molecules. Transient and stable expression of the ZmCPK4-GFP fusion proteins revealed ZmCPK4 localized to the membrane. Moreover, overexpression of ZmCPK4 in the transgenic Arabidopsis enhanced ABA sensitivity in seed germination, seedling growth and stomatal movement. The transgenic plants also enhanced drought stress tolerance. Taken together, the results suggest that ZmCPK4 might be involved in ABA-mediated regulation of stomatal closure in response to drought stress.
钙依赖蛋白激酶（CDPKs）在植物应对非生物胁迫的钙介导信号转导中发挥重要作用。已经鉴定出一些成员是植物对脱落酸（ABA）信号响应的调节剂。在这里，我们从玉米中分离出一个亚组 I CDPK 基因，ZmCPK4。定量实时 PCR（qRT-PCR）分析显示，ZmCPK4 转录物受各种胁迫和信号分子的诱导。ZmCPK4-GFP 融合蛋白的瞬时和稳定表达表明 ZmCPK4 定位于膜上。此外，在转基因拟南芥中过表达 ZmCPK4 增强了 ABA 对种子萌发、幼苗生长和气孔运动的敏感性。转基因植物也增强了抗旱性。综上所述，结果表明 ZmCPK4 可能参与 ABA 介导的气孔关闭对干旱胁迫的调节。
