Department of Small Animal, Universidade Federal de Santa Maria, Brazil.
Exp Parasitol. 2013 Oct;135(2):325-30. doi: 10.1016/j.exppara.2013.07.014. Epub 2013 Jul 30.
An investigation of E-NTPDase and E-ADA activities in lymphocytes from rats experimentally infected with Toxoplasma gondii was carried out in this study. For this purpose, twenty four adult male Wistar rats were divided in two groups/four subgroups (A1 and A2; B1 and B2-6 animal/each group), with "A" as uninfected and "B" inoculated with T. gondii (RH strain). Sampling was performed on days 5 and 10 post-infection (p.i.), with evaluation of hemogram, immunoglobulins (IgM and IgG) and activity of E-NTPDase and E-ADA in lymphocytes. Enzymes essays showed ATP hydrolysis increased on days 5 (P<0.05) and 10 (P<0.01) p.i., as well as an increase of ADP hydrolysis on day 10 (P<0.01) p.i. E-ADA activity on lymphocytes was also increased in both evaluated periods (P<0.01). Based on E-NTPDase and E-ADA increased activities observed on lymphocytes, it is possible to suggest their involvement in an anti-inflammatory response, consisting of a modulatory response, preventing excessive tissue damage caused by the infection with Toxoplasma gondii.
本研究旨在调查实验感染弓形虫的大鼠淋巴细胞中的 E-NTPDase 和 E-ADA 活性。为此，将 24 只成年雄性 Wistar 大鼠分为两组/四组亚组（A1 和 A2；B1 和 B2-6 只动物/每组），其中“A”为未感染，“B”为接种 T. gondii（RH 株）。感染后第 5 和第 10 天进行采样，评估血象、免疫球蛋白（IgM 和 IgG）以及淋巴细胞中 E-NTPDase 和 E-ADA 的活性。酶谱分析显示，感染后第 5 天（P<0.05）和第 10 天（P<0.01）ATP 水解增加，第 10 天（P<0.01）ADP 水解增加。在两个评估期内，淋巴细胞中的 E-ADA 活性也增加（P<0.01）。基于观察到的淋巴细胞中 E-NTPDase 和 E-ADA 活性的增加，可以推测它们参与了抗炎反应，包括调节反应，以防止弓形虫感染引起的过度组织损伤。
