School of Chemistry and Chemical Engineering, Southwest University, Chongqing, Beibei 400715, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2013 Dec;116:57-64. doi: 10.1016/j.saa.2013.06.018. Epub 2013 Jul 16.
The interaction of mebendazole (MBZ) with 12-tungstophosphoric acid (TP) has been investigated by using resonance Rayleigh scattering (RRS) and frequency doubling scattering (FDS) combining with absorption spectrum. In pH 1.0 HCl medium, MBZ reacted with TP to form 3:1 ion-association complex. As a result, not only the spectrum of absorption was changed, but also the intensities of RRS and FDS were enhanced greatly. The maximum RRS, FDS and absorption wavelengths are located at 372, 392 and 260 nm, respectively. The increments of scattering intensity (ΔI) and absorption (ΔA) are directly proportional to the concentrations of MBZ in certain ranges. The detection limits (3σ) of RRS, FDS and absorption are 0.56, 0.86 and 130.16 ng/mL, respectively. The sensitivity of RRS method is higher than FDS and absorption methods. The optimum conditions of RRS method and the influence factors were discussed in the paper, in addition, the structure of ion-association complex and the reaction mechanism were investigated. Based on the ion-association reaction and its spectral response, the rapid, simple and sensitive RRS method for the determination of MBZ has been developed.
苯并咪唑（MBZ）与 12-钨磷酸（TP）的相互作用通过共振瑞利散射（RRS）和倍频散射（FDS）与吸收光谱结合进行了研究。在 pH 1.0 HCl 介质中，MBZ 与 TP 反应形成 3:1 的离子缔合配合物。结果不仅改变了吸收光谱，而且大大增强了 RRS 和 FDS 的强度。最大 RRS、FDS 和吸收波长分别位于 372、392 和 260nm。散射强度（ΔI）和吸收（ΔA）的增量与 MBZ 在一定范围内的浓度成正比。RRS、FDS 和吸收的检测限（3σ）分别为 0.56、0.86 和 130.16ng/mL。RRS 方法的灵敏度高于 FDS 和吸收方法。本文讨论了 RRS 方法的最佳条件和影响因素，此外，还研究了离子缔合配合物的结构和反应机制。基于离子缔合反应及其光谱响应，建立了一种快速、简单、灵敏的 RRS 法测定 MBZ。
