共振瑞利散射光谱、孔雀石绿-12-磷钨酸体系的非线性散射光谱及其在鱼类中的分析应用。
Resonance Rayleigh scattering spectra, non-linear scattering spectra of malachite green-12-tungstophosphoric acid system and its analytical application in fish.
机构信息
School of Chemistry and Chemical Engineering, Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, Southwest University, Chongqing 400715, China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2011 Jan;78(1):148-52. doi: 10.1016/j.saa.2010.09.014. Epub 2010 Sep 17.
In 0.1 molL(-1) (pH 1.0) HCl medium, 12-tungstophosphoric acid (TP) reacted with malachite green (MG) to form an ion-association complex. As a result, the new spectra of RRS, SOS and FDS appeared and their intensities were enhanced greatly. The maximum wavelengths of RRS, SOS and FDS were located at 334 nm, 586 nm and 330 nm, and the scattering intensities were proportional to the concentration of MG. Based on it a new method for the determination of MG has been established. The detection limits (3σ) of these methods were in the range of 3.7-27 ng mL(-1). The RRS, SOS, and FDS characteristics, absorption spectrum characteristics and optimum reaction conditions of the system were discussed. Effects of coexistent substances were tested, and the results demonstrated that this method had good selectivity. It has been applied to the determination of malachite green residues in fish flesh with satisfactory results. The reaction mechanism and reasons of RRS enhancement are discussed.
在 0.1 molL(-1) (pH 1.0) HCl 介质中，十二钨磷酸 (TP) 与孔雀石绿 (MG) 反应形成离子缔合物。结果，新的 RRS、SOS 和 FDS 光谱出现，其强度大大增强。RRS、SOS 和 FDS 的最大波长分别位于 334nm、586nm 和 330nm，散射强度与 MG 的浓度成正比。基于此，建立了一种测定 MG 的新方法。这些方法的检测限 (3σ) 在 3.7-27ngmL(-1)范围内。讨论了体系的 RRS、SOS 和 FDS 特性、吸收光谱特性和最佳反应条件。测试了共存物质的影响，结果表明该方法具有良好的选择性。已将其应用于鱼肉中孔雀石绿残留量的测定，结果令人满意。讨论了 RRS 增强的反应机制和原因。
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