Departamento de Ciências Patológicas, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Campus Universitário, 86057-970, Londrina, Paraná, Brazil.
Mycopathologia. 2013 Oct;176(3-4):299-302. doi: 10.1007/s11046-013-9681-9. Epub 2013 Aug 4.
The objective of the present study was to evaluate infection of cats by Paracoccidioides brasiliensis. Serum samples of 136 cats from rural (n = 86) and urban areas (n = 50) were analyzed by indirect ELISA and immunodiffusion test using P. brasiliensis gp43 and exoantigen as antigens, respectively, and an overall reactivity of 31.6 % was observed by ELISA although no reactivity was detected by immunodiffusion. The positivity observed in animals living in rural areas (48.8 %) with free access to soil was significantly higher (P < 0.0001) than among urban animals (2 %) with limited access to soil, although no significant difference was observed in relation to age or sex. The high rates of positivity observed in cats from rural areas suggest that not diagnosed cases of this mycosis may be occurring in cats living in endemic areas for human paracoccidioidomycosis. This is the first report showing serological evidence of P. brasiliensis infection in cats.
本研究旨在评估巴西副球孢子菌对猫的感染。采用间接 ELISA 和免疫扩散试验，分别使用 P. brasiliensis gp43 和外抗原作为抗原，对来自农村（n=86）和城市（n=50）地区的 136 只猫的血清样本进行分析，ELISA 观察到总反应率为 31.6%，但免疫扩散试验未检测到反应。在可以自由接触土壤的农村地区动物中观察到的阳性率（48.8%）明显高于接触土壤有限的城市动物（2%）（P<0.0001），但与年龄或性别无关。农村地区猫的高阳性率表明，在人副球孢子菌病流行地区生活的猫可能存在未确诊的此类真菌感染病例。这是首次报道猫巴西副球孢子菌感染的血清学证据。
