Silveira L H, Paes R C S, Medeiros E V, Itano E N, Camargo Z P, Ono M A
Departamento de Ciências Patológicas, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Campus Universitário, Londrina, PR, Brazil.
Mycopathologia. 2008 Jun;165(6):367-71. doi: 10.1007/s11046-008-9095-2. Epub 2008 Mar 5.
The aim of this study was to evaluate the humoral immune response in cattle immunized with Paracoccidioides brasiliensis and perform a seroepidemiological study of paracoccidioidomycosis in dairy cattle from Mato Grosso do Sul. Two animals (one steer and one heifer) were inoculated with a suspension of P. brasiliensis in Freund incomplete adjuvant. Blood samples were collected periodically to evaluate humoral immune response by immunodiffusion and ELISA, using exoantigen and gp43 as antigens, respectively. The antibody production was detected by immunodiffusion and ELISA, in both animals, 14 days after immunization. The soroepidemiologic study was carried out in 400 cattle of Mato Grosso do Sul from four municipalities: Corumbá, Dourados, Nova Andradina, and São Gabriel d'Oeste. The municipalities of Corumbá (30%) and Nova Andradina (28%) showed higher positivity than Dourados (8%) and São Gabriel d'Oeste (4%). In this study we concluded that cattle immunized with P. brasiliensis develop humoral immune response for gp43, remaining with high titers of antibodies, and that this animal species could be an epidemiologic marker of paracoccidioidomycosis.
本研究的目的是评估用巴西副球孢子菌免疫的牛的体液免疫反应，并对南马托格罗索州奶牛的副球孢子菌病进行血清流行病学研究。用巴西副球孢子菌悬浮液与弗氏不完全佐剂接种两只动物（一头公牛和一头小母牛）。定期采集血样，分别使用外抗原和gp43作为抗原，通过免疫扩散和ELISA评估体液免疫反应。免疫14天后，通过免疫扩散和ELISA在两只动物中均检测到抗体产生。血清流行病学研究在南马托格罗索州四个市（科伦巴、多拉杜斯、新安德拉迪纳和西圣加布里埃尔）的400头牛中进行。科伦巴市（30%）和新安德拉迪纳市（28%）的阳性率高于多拉杜斯市（8%）和西圣加布里埃尔市（4%）。在本研究中，我们得出结论，用巴西副球孢子菌免疫的牛对gp43产生体液免疫反应，抗体水平维持在较高水平，并且该动物物种可能是副球孢子菌病的流行病学标志物。
