Kaplan Ozgür, Meriç Murat, Acar Zeydin, Kale Abdurrahman, Demircan Sabri, Yılmaz Ozcan, Demircan Günnur, Yılmaz Miroğlu Yeliz
Department of Cardiology Faculty of Medicine, Ondokuz Mayıs University, Samsun-Turkey.
Anadolu Kardiyol Derg. 2013 Nov;13(7):641-6. doi: 10.5152/akd.2013.186. Epub 2013 Jul 31.
In this study the antioxidant enzyme [catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) ] levels at rest in patients with syndrome X and coronary slow flow are measured. Then it has been investigated whether there is any enzymatic difference between the normal controls and syndrome X patients or patients with coronary slow flow and ascertain if exercise has any effects on the antioxidant enzyme levels.
Fifty-five patients were included in this prospective observational controlled study. Patients were divided into 3 groups: Group 1- normal controls (n=20); Group 2-patients with coronary slow flow (n=20); and Group 3-patients diagnosed with syndrome X (n=15). In all patients, blood samples were collected at rest and after maximal exercise. The antioxidant enzymes (SOD, CAT, Gpx) in the erythrocytes were studied for these three groups of blood sample. Statistical analysis was performed using Student t-test, Mann-Whitney U and Chi-square tests, Kruskal-Wallis variance analysis and ANOVA.
Under basal conditions the lowest SOD and GPx levels were measured in the 2nd Group, whereas significant differences in paired comparisons were observed only between the 2nd and 3rd Groups (p=0.024 vs. p<0.01, respectively) during paired comparisons. The post-exercise SOD levels were decreased significantly in the 3rd Groups when compared with the basal concentrations (p=0.014), however no significant pre- and post-exercise differences were observed in the CAT and GPx concentrations (p>0.05).
The post-exercise SOD level when compared with basal SOD levels were decreased significantly in the syndrome X group, however no differences were observed between the other groups. This can be interpreted as the reduction in the exercise related symptoms and ischemic findings are resulting from the decrease of SOD activity.
本研究测量了X综合征和冠状动脉血流缓慢患者静息状态下的抗氧化酶[过氧化氢酶（CAT）、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPx）]水平。然后研究了正常对照组与X综合征患者或冠状动脉血流缓慢患者之间是否存在酶学差异，并确定运动是否对抗氧化酶水平有任何影响。
55例患者纳入本前瞻性观察性对照研究。患者分为3组：第1组为正常对照组（n = 20）；第2组为冠状动脉血流缓慢患者（n = 20）；第3组为诊断为X综合征的患者（n = 15）。所有患者在静息状态和最大运动后采集血样。对这三组血样研究红细胞中的抗氧化酶（SOD、CAT、Gpx）。采用学生t检验、曼-惠特尼U检验和卡方检验、克鲁斯卡尔-沃利斯方差分析和方差分析进行统计分析。
在基础条件下，第2组测量到最低的SOD和GPx水平，而在配对比较中，仅在第2组和第3组之间观察到显著差异（分别为p = 0.024和p < 0.01）。与基础浓度相比，第3组运动后SOD水平显著降低（p = 0.014），然而，CAT和GPx浓度在运动前后未观察到显著差异（p > 0.05）。
与基础SOD水平相比，X综合征组运动后SOD水平显著降低，然而其他组之间未观察到差异。这可以解释为运动相关症状和缺血表现的减轻是由于SOD活性降低所致。
