拟南芥异三聚体G蛋白突变体下胚轴中细胞分裂和细胞伸长的分析

Analysis of cell division and cell elongation in the hypocotyls of Arabidopsis heterotrimeric G protein mutants.

作者信息

Jin Zhaoqing, Muchero Wellington, Chen Jin-Gui

机构信息

Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.

出版信息

Methods Mol Biol. 2013;1043:37-43. doi: 10.1007/978-1-62703-532-3_4.

DOI:10.1007/978-1-62703-532-3_4

PMID:23913033

Abstract

The heterotrimeric G-proteins form classical signal transduction complexes conserved in all eukaryotes. The repertoire of G-protein signaling complex is much simpler in plants than in metazoans. One of the best understood functions for the plant G-protein complex is its modulation of cell proliferation. A method is described here to quantify cell division and cell elongation in the Arabidopsis heterotrimeric G-protein mutants using the hypocotyl as a model system.

摘要

异源三聚体G蛋白形成了在所有真核生物中都保守的经典信号转导复合物。植物中G蛋白信号复合物的种类比后生动物中的要简单得多。植物G蛋白复合物最广为人知的功能之一是其对细胞增殖的调节作用。本文描述了一种以拟南芥下胚轴为模型系统，对拟南芥异源三聚体G蛋白突变体中的细胞分裂和细胞伸长进行定量的方法。