Wu Wenjuan, Huang Jirong
National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
Methods Mol Biol. 2013;1043:81-7. doi: 10.1007/978-1-62703-532-3_9.
Functional analysis of G-proteins has been extensively carried out using their over-expressing lines and knockout mutants in plants. Since α subunit exists in an active or inactive form, overexpressing α subunit does not mean that G-protein signaling pathways are activated in the transgenic lines. Ectopic expression of the constitutively active form of the α subunit will magnify a role of G-protein signaling pathways in plant growth and development, and ultimately yield phenotypes. Here, we describe the method to study function of G-proteins in chloroplast development using the constitutively active form of the α subunit in Arabidopsis.
利用植物中G蛋白的过表达株系和敲除突变体，已经对其进行了广泛的功能分析。由于α亚基以活性或非活性形式存在，过表达α亚基并不意味着G蛋白信号通路在转基因株系中被激活。α亚基组成型活性形式的异位表达将放大G蛋白信号通路在植物生长发育中的作用，并最终产生表型。在这里，我们描述了利用拟南芥中α亚基的组成型活性形式来研究G蛋白在叶绿体发育中的功能的方法。
