Trusov Yuri, Chakravorty David, Botella Jose Ramon
Plant Genetic Engineering Laboratory, School of Agriculture and Food Sciences, University of Queensland, Brisbane, QLD, Australia.
Methods Mol Biol. 2013;1043:67-72. doi: 10.1007/978-1-62703-532-3_7.
Increased susceptibility to Fusarium oxysporum is one of the most conspicuous characteristics of the Arabidopsis mutants lacking the heterotrimeric G protein β and γ1 subunits. The molecular mechanisms placing these G proteins in the plant innate immunity network are yet to be discovered. However, a method to test susceptibility to and disease progression of an important plant pathogen, such as F. oxysporum, is of central importance to many plant defense studies. The optimized protocol presented here allows the routine processing and analysis of symptom progression in young Arabidopsis soil-grown seedlings and yields highly reproducible results.
对尖孢镰刀菌易感性增加是缺乏异源三聚体G蛋白β和γ1亚基的拟南芥突变体最显著的特征之一。将这些G蛋白置于植物先天免疫网络中的分子机制尚待发现。然而，一种测试对重要植物病原体（如尖孢镰刀菌）的易感性和疾病进展的方法对许多植物防御研究至关重要。本文介绍的优化方案允许对在土壤中生长的拟南芥幼苗的症状进展进行常规处理和分析，并产生高度可重复的结果。
