Yang Lijuan, Lai Dongmei
The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
Methods Mol Biol. 2013;1049:337-45. doi: 10.1007/978-1-62703-547-7_25.
The concept of cancer stem cells (CSCs) provides a new paradigm for understanding cancer biology. Cancer stem cells are defined as a minority of cancer cells with stem cell properties responsible for maintenance and growth of tumors. The targeting of CSCs is a potential therapeutic strategy to combat ovarian cancer. Ovarian epithelial cancer cells cultured in serum-free medium can form sphere cells. These sphere cells may be enriched for cancer stem cells (CSCs). The isolation of sphere cells from solid tumors is an important technique in studying cancer cell biology. Here we describe the isolation of sphere cells from primary ovarian cancer tissue, ascites fluid, and the cancer cell line SKOV3 with stem cell selection medium.
癌症干细胞（CSCs）的概念为理解癌症生物学提供了一种新的范例。癌症干细胞被定义为具有干细胞特性的少数癌细胞，它们负责肿瘤的维持和生长。靶向癌症干细胞是对抗卵巢癌的一种潜在治疗策略。在无血清培养基中培养的卵巢上皮癌细胞可以形成球形细胞。这些球形细胞可能富含癌症干细胞（CSCs）。从实体瘤中分离球形细胞是研究癌细胞生物学的一项重要技术。在这里，我们描述了使用干细胞选择培养基从原发性卵巢癌组织、腹水和癌细胞系SKOV3中分离球形细胞的方法。
