Roberts Gareth A, Dryden David T F
School of Chemistry, University of Edinburgh, Edinburgh, UK.
Methods Mol Biol. 2013;1054:1-9. doi: 10.1007/978-1-62703-565-1_1.
The technique of gel electrophoresis is now firmly established as a routine laboratory method for analyzing DNA. Here, we describe the development of the methodology as well as a brief explanation of how the technique works. There is a short introduction to pulsed-field agarose gel electrophoresis, which represents a critical advancement in the method that facilitates the analysis of very large fragments of DNA. Finally, theoretical considerations are included.
凝胶电泳技术现已成为分析DNA的常规实验室方法。在此，我们描述该方法的发展以及对该技术工作原理的简要解释。还简要介绍了脉冲场琼脂糖凝胶电泳，它是该方法的一项关键进展，有助于分析非常大的DNA片段。最后，还包括理论方面的考量。
