Du X Y, Hu Q N, Zhang Q L, Wang Y B, Luo Z R
Institute of Horticultural Sciences, Jiangxi Academy of Agricultural Sciences, Nanchang, P.R. China.
Genet Mol Res. 2013 Jun 6;12(2):1781-95. doi: 10.4238/2013.June.6.2.
Retrotransposon-based molecular markers are powerful molecular tools. However, these markers are not readily available due to the difficulty in obtaining species-specific retrotransposon primers. Although recent techniques enabling the rapid isolation of retrotransposon sequences have facilitated primer development, this process nonetheless remains time-consuming and costly. Therefore, research into the transferability of retrotransposon primers developed from one plant species onto others would be of great value. The present study investigated the transferability of retrotransposon primers derived from 'Luotian-tianshi' persimmon (Diospyros kaki Thunb.) across other fruit crops, as well as within the genus using inter-retrotransposon amplified polymorphism molecular marker. Fourteen of the 26 retrotransposon primers tested (53.85%) produced robust and reproducible amplification products across all fruit crops tested, indicating their applicability across plant species. Four of the 13 fruit crops showed the best transferability performances: persimmon, grape, citrus, and peach. Furthermore, similarity coefficients and UPGMA clustering indicated that these primers could further offer a potential tool for germplasm differentiation, parentage identification, genetic diversity assessment, classification, and phylogenetic studies across a variety of plant species. Transferability was further confirmed by examining published primers derived from Rosaceae, Gramineae, and Solanaceae. This study is one of the few currently available studies concerning the transferability of retrotransposon primers across plant species in general, and is the first successful study of the transferability of retrotransposon primers derived from persimmon. The primers presented here will help reduce costs for future retrotransposon primer development and therefore contribute to the popularization of retrotransposon molecular markers.
基于反转录转座子的分子标记是强大的分子工具。然而，由于难以获得物种特异性的反转录转座子引物，这些标记并不容易获得。尽管最近能够快速分离反转录转座子序列的技术促进了引物的开发，但这个过程仍然耗时且成本高昂。因此，研究从一种植物物种开发的反转录转座子引物在其他物种上的转移性具有重要价值。本研究使用反转录转座子间扩增多态性分子标记，研究了源自‘罗田甜柿’（Diospyros kaki Thunb.）的反转录转座子引物在其他水果作物以及柿属内的转移性。在测试的26个反转录转座子引物中，有14个（53.85%）在所有测试的水果作物中产生了稳定且可重复的扩增产物，表明它们在不同植物物种中的适用性。13种水果作物中有4种表现出最佳的转移性：柿子、葡萄、柑橘和桃子。此外，相似系数和UPGMA聚类分析表明，这些引物可以进一步为各种植物物种的种质分化、亲本鉴定、遗传多样性评估、分类和系统发育研究提供潜在工具。通过检查源自蔷薇科、禾本科和茄科的已发表引物，进一步证实了转移性。本研究是目前为数不多的关于反转录转座子引物在植物物种间转移性的研究之一，也是关于柿子来源的反转录转座子引物转移性的首次成功研究。这里展示的引物将有助于降低未来反转录转座子引物开发的成本，从而有助于反转录转座子分子标记的推广。
