Abbasi Holasou Hossein, Valizadeh Negar, Mohammadi Seyyed Abolghasem
Laboratory of Genomics and Molecular Plant Breeding, Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz, 5166614766 Iran.
Department of Life Sciences, Center for Cell Pathology, Khazar University, Baku, AZ1096 Azerbaijan.
Rev Bras Bot. 2023;46(1):51-60. doi: 10.1007/s40415-022-00860-x. Epub 2023 Jan 3.
The knowledge about the level of genetic diversity and population structure in natural populations of L. is a primary step in breeding programs for development of new cultivars with higher artemisinin level and better quality of secondary metabolites composition. We used PCR-based "retrotransposon-microsatellite amplified polymorphisms" (REMAPs) to study insertional polymorphism in genome to assess genetic variability and population structure in a collection of 118 accessions collected from north and northwest of Iran. Twenty-five primer combinations of 10 retrotransposon and seven ISSR primers amplified a total of 693 clear and unambiguous fragments in the studied accessions. The average number of bands, polymorphic bands, polymorphism, effective number of alleles, Shannon's information index and expected heterozygosity were 27.72, 24.76, 88.14%, 1.47, 0.42 and 0.28, respectively. The analysis of molecular variance revealed high genetic variation present within sampled geographical regions. Distance-based cluster analysis assigned the studied accessions into four clusters according to their geographical origin, which were also confirmed by principal coordinate analysis. In model-based Bayesian clustering, the maximum value of Δ was obtained when the collection of 118 assayed accessions assigned into two subgroups ( = 2). The results showed the high genetic variation in the collection of Iranian sweet wormwood which revealed by REMAP markers indicating the reliability and efficiency of this marker system for analysis of genetic diversity and population structure of .
The online version contains supplementary material available at 10.1007/s40415-022-00860-x.
了解黄花蒿自然种群的遗传多样性水平和种群结构，是培育青蒿素含量更高、次生代谢产物组成质量更好的新品种育种计划的首要步骤。我们使用基于PCR的“反转录转座子-微卫星扩增多态性”（REMAPs）来研究基因组中的插入多态性，以评估从伊朗北部和西北部收集的118份材料的遗传变异性和种群结构。10个反转录转座子的25对引物组合和7对ISSR引物在研究的材料中总共扩增出693条清晰且明确的条带。条带平均数、多态性条带数、多态性、有效等位基因数、香农信息指数和期望杂合度分别为27.72、24.76、88.14%、1.47、0.42和0.28。分子方差分析显示，在采样的地理区域内存在高度的遗传变异。基于距离的聚类分析根据地理起源将研究的材料分为四个聚类，主坐标分析也证实了这一点。在基于模型的贝叶斯聚类中，当将118份测定材料分为两个亚组（K = 2）时，获得了Δ的最大值。结果表明，REMAP标记揭示了伊朗黄花蒿材料中存在高度的遗传变异，表明该标记系统在分析黄花蒿遗传多样性和种群结构方面的可靠性和有效性。
在线版本包含可在10.1007/s40415-022-00860-x获取的补充材料。
