Nuclear Fuel Cycle Research School, Nuclear Science & Technology Research Institute, Atomic Energy Organization of Iran, Tehran, Iran.
J Sep Sci. 2013 Aug;36(16):2629-34. doi: 10.1002/jssc.201300356.
A new pretreatment method, SPE combined with dispersive liquid-liquid microextraction, was proposed for the determination of abamectin in citrus fruit samples for the first time. In this method, fruit samples were extracted by ultrasound-assisted extraction followed by SPE. Then, the SPE was used as a disperser solvent in the next dispersive liquid-liquid microextraction step for further purification and enrichment of abamectin. The effects of various parameters on the extraction efficiency of the proposed method were investigated and optimized. Good linearity of abamectin was obtained from 0.005 to 10.0 mg/kg for B1a and from 0.05 to 10.0 mg/kg for B1b with correlation coefficient (r(2)) of 0.998 for B1a and 0.991 for B1b, respectively. The LODs were 0.001 and 0.008 mg/kg (S/N = 3) for B1a and B1b, respectively. The relative recoveries at three spiked levels were ranged from 87 to 96% with the RSD less than 11% (n = 3). The method has been successfully applied to the determination of abamectin in citrus fruit samples.
一种新的预处理方法，即固相萃取（SPE）结合分散液液微萃取（DLLME），首次被用于柑橘类水果样品中阿维菌素的测定。在该方法中，水果样品先通过超声辅助提取，然后再通过 SPE 进行提取。接下来，SPE 作为分散溶剂，用于下一步的分散液液微萃取，以进一步对阿维菌素进行纯化和富集。考察并优化了各种参数对该方法萃取效率的影响。阿维菌素 B1a 的线性范围为 0.005 至 10.0 mg/kg，相关系数（r(2)）为 0.998；阿维菌素 B1b 的线性范围为 0.05 至 10.0 mg/kg，相关系数（r(2)）为 0.991。B1a 和 B1b 的检出限分别为 0.001 和 0.008 mg/kg（S/N = 3）。在三个加标水平下的相对回收率在 87%至 96%之间，相对标准偏差（RSD）小于 11%（n = 3）。该方法已成功应用于柑橘类水果样品中阿维菌素的测定。
