Pawlik Justyna, Widziołek Magdalena, Cholewa-Kowalska Katarzyna, Łączka Maria, Osyczka Anna Maria
Department of Glass Technology and Amorphous Coatings, Faculty of Materials Engineering and Ceramics, University of Science and Technology, Krakow, Poland.
J Biomed Mater Res A. 2014 Jul;102(7):2383-94. doi: 10.1002/jbm.a.34903. Epub 2013 Sep 12.
We developed TiO2 matrix composites modified by sol-gel bioactive glasses (SBG) of either high CaO content (A2) or high SiO2 content (S2). The latter were mixed with titanium dioxide (TiO2) at 75:25, 50:50, and 25:75 weight ratios and sintered at 1250°C for 2 h. We examined the effects of various types (A2 or S2) and compositional TiO2 :SBG ratios on the mechanical properties of resulting composites, their bioactivity and human bone marrow mesenchymal stem cells (MSC) response. The chemistry of SBGs influenced the phase composition, mechanical and biological properties of the composites. Rutile and titanite prevailed in A2-TiO2 composites, and rutile and crystobalite in S2-TiO2 composites. Compressive strength increased significantly for 25A2-TiO2 composites (140 MPa) compared to matrix TiO2 (58 MPa). Composites containing 50-75 wt % of either SBG displayed bioactive properties as determined by simulated body fluid test. Compared to TiO2, human bone marrow stromal cell (BMSC) viability was enhanced on the composites containing 25 wt % of either SBG, whereas the composites modified by 25 wt % of S2 enhanced alkaline phosphatase activity and mineralization in cultures treated with osteogenic inducers-dexamethasone (Dex) or bone morphogenetic protein. Increasing amounts of A2 in TiO2 matrix decreased cell viability but increased collagen deposition and mineralized matrix production by BMSC. Considering the physico-chemical and biological properties of the presented composites, the modification of TiO2 with SBG may prove useful strategy in several bone tissue related regeneration strategies.
我们开发了由高CaO含量（A2）或高SiO2含量（S2）的溶胶 - 凝胶生物活性玻璃（SBG）改性的TiO2基复合材料。将后者与二氧化钛（TiO2）按75:25、50:50和25:75的重量比混合，并在1250°C下烧结2小时。我们研究了不同类型（A2或S2）以及TiO2:SBG组成比例对所得复合材料的力学性能、生物活性和人骨髓间充质干细胞（MSC）反应的影响。SBG的化学性质影响了复合材料的相组成、力学和生物学性能。金红石和钛酸钙在A2 - TiO2复合材料中占主导，而金红石和方石英在S2 - TiO2复合材料中占主导。与基体TiO2（58 MPa）相比，25A2 - TiO2复合材料的抗压强度显著提高（140 MPa）。通过模拟体液试验确定，含有50 - 75 wt%任何一种SBG的复合材料都具有生物活性。与TiO2相比，含有25 wt%任何一种SBG的复合材料上的人骨髓基质细胞（BMSC）活力增强，而用25 wt% S2改性的复合材料在用成骨诱导剂 - 地塞米松（Dex）或骨形态发生蛋白处理的培养物中增强了碱性磷酸酶活性和矿化作用。TiO2基体中A2含量的增加降低了细胞活力，但增加了BMSC的胶原蛋白沉积和矿化基质生成。考虑到所呈现复合材料的物理化学和生物学性质，用SBG对TiO2进行改性可能是几种骨组织相关再生策略中的有用策略。
