Department Biology and Cell Imaging, Institute of Zoology and Biomedical Research, Faculty of Biology, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, Poland.
Department of Glass Technology and Amorphous Coatings, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Mickiewicza Ave. 30, 30-059 Krakow, Poland.
Int J Mol Sci. 2022 Feb 26;23(5):2598. doi: 10.3390/ijms23052598.
In this work, a poly(L-lactide--glycolide) (PLGA)-based composite was enriched with one of the following sol-gel bioactive glasses (SBG) at 50 wt.%: A1-40 mol% SiO, 60 mol% CaO, CaO/SiO ratio of 1.50; S1-80 mol% SiO, 20 mol% CaO, CaO/SiO ratio of 0.25; A2-40 mol% SiO, 54 mol% CaO, 6 mol% PO, CaO/SiO ratio of 1.35; S2-80 mol% SiO,16 mol% CaO, 4 mol% PO, CaO/SiO ratio of 0.20. The composites and PLGA control sheets were then soaked for 24 h in culture media, and the obtained condition media (CM) were used to treat human bone marrow stromal cells (hBMSCs) for 72 h. All CMs from the composites increased ERK 1/2 activity vs. the control PLGA CM. However, expressions of cell migration-related c-Fos, osteopontin, matrix metalloproteinase-2, C-X-C chemokine receptor type 4, vascular endothelial growth factor, and bone morphogenetic protein 2 were significantly increased only in cells treated with the CM from the A1/PLGA composite. This CM also significantly increased the rate of human BMSC migration but did not affect cell metabolic activity. These results indicate important biological markers that are upregulated by products released from the bioactive composites of a specific chemical composition, which may eventually prompt osteoprogenitor cells to colonize the bioactive material and accelerate the process of tissue regeneration.
在这项工作中，将聚（L-丙交酯-乙交酯）（PLGA）基复合材料用以下溶胶-凝胶生物活性玻璃（SBG）之一以 50wt%进行富集：A1-40mol%SiO，60mol%CaO，CaO/SiO 比为 1.50；S1-80mol%SiO，20mol%CaO，CaO/SiO 比为 0.25；A2-40mol%SiO，54mol%CaO，6mol%PO，CaO/SiO 比为 1.35；S2-80mol%SiO，16mol%CaO，4mol%PO，CaO/SiO 比为 0.20。然后将复合材料和 PLGA 对照片在培养基中浸泡 24 小时，并用获得的条件培养基（CM）处理人骨髓基质细胞（hBMSCs）72 小时。所有来自复合材料的 CM 均增加了 ERK1/2 活性，而对照 PLGA CM 则增加了 ERK1/2 活性。然而，细胞迁移相关的 c-Fos、骨桥蛋白、基质金属蛋白酶-2、C-X-C 趋化因子受体 4、血管内皮生长因子和骨形态发生蛋白 2 的表达仅在经 A1/PLGA 复合材料 CM 处理的细胞中显著增加。该 CM 还显著增加了人 BMSC 的迁移率，但不影响细胞代谢活性。这些结果表明，由特定化学成分的生物活性复合材料释放的产物上调了重要的生物学标志物，这可能最终促使成骨前体细胞定植于生物活性材料并加速组织再生过程。
