Cai Wei-Xin, Zheng Li-Wu, Weber Franz E, Li Chun-Lei, Ma Li, Ehrbar Martin, Zwahlen Roger A
Discipline of Oral & Maxillofacial Surgery, Faculty of Dentistry, The University of Hong Kong , Hong Kong, China .
Biores Open Access. 2013 Aug;2(4):266-72. doi: 10.1089/biores.2013.0025.
To achieve an easily established, safe, and reproducible animal model for the study of heterotopic bone formation around vessels, a small animal series using New Zealand White rabbits was performed. Three different dosages of recombinant human bone morphogenic protein (rhBMP-2) carried by fibrin matrix were tested. A guided tissue regeneration (GTR) membrane sheet was formed into a tube and allowed to harden; it served both to maintain the space around the vessel bundle and to separate the fibrin matrix with rhBMP-2 from skeletal muscle. Wrapped around the femoral vessel bundle and fixed in place, the tube was filled with the fibrin matrix containing rhBMP-2. The surgical site was closed in layers, and the postoperative healing was uneventful. All animals resumed their full preoperative daily activities 3-4 days after the operation. No adverse events such as wound dehiscence or infection occurred, and all animals could be sacrified at the scheduled date. Micro-computed tomography and histological investigations showed heterotopic bone formation around the vessel bundle in the medium- and high-dosage rhBMP-2 groups. An easy, safe, and reproducible animal model that allows the study of heterotopic bone formation around vessels was successfully established.
为建立一种易于构建、安全且可重复的用于研究血管周围异位骨形成的动物模型，我们使用新西兰白兔进行了一系列小动物实验。测试了由纤维蛋白基质携带的三种不同剂量的重组人骨形态发生蛋白（rhBMP-2）。将引导组织再生（GTR）膜片制成管状并使其硬化；它既用于维持血管束周围的空间，又用于将含有rhBMP-2的纤维蛋白基质与骨骼肌分开。将该管包裹在股血管束周围并固定到位，然后用含有rhBMP-2的纤维蛋白基质填充。手术部位分层缝合，术后愈合顺利。所有动物在术后3-4天恢复了术前的全部日常活动。未发生伤口裂开或感染等不良事件，所有动物均可在预定日期处死。微计算机断层扫描和组织学研究显示，中高剂量rhBMP-2组的血管束周围有异位骨形成。成功建立了一种简单、安全且可重复的动物模型，可用于研究血管周围的异位骨形成。
