Department of Biochemical Research, Instituto de Investigación Sanitaria Fundación Jiménez Díaz , Madrid , Spain .
Aging Male. 2014 Mar;17(1):42-50. doi: 10.3109/13685538.2013.821697. Epub 2013 Aug 5.
The purpose of this study was to evaluate the effect of parathyroid hormone (PTH) (1-84) in a model of male osteoporosis induced by orchidectomy in rats.
Six-month-old Wistar rats were used as follows: SHAM (simulated orchidectomy), orchidectomized (ORX), ORX + PTH1 (ORX and treated with 10 µg/Kg/d of PTH 1-84) and ORX + PTH2 (ORX and treated with 50 µg/Kg/d of PTH 1-84) over 3 months, with treatment beginning three months after orchidectomy.
Orchidectomy resulted in a decreased of femoral and lumbar bone mineral density (BMD), a worsening of trabecular and cortical microarchitecture and a decrease in biomechanical properties. Both doses of PTH (1-84) partially (low dose) or totally (high dose) restored the ORX-induced changes. Serum C-telopeptide of type I collagen/5b isoenzyme of tartrate-resistant acid phosphatase (CTX/TRAP) resorption index increased after orchidectomy. Osteocalcin (bone Gla protein; BGP) levels were not affected by orchidectomy. PTH (1-84) treatment did not produce any changes in the levels of CTX/TRAP with respect to the ORX group. BGP levels increased with PTH treatment.
PTH (1-84) is able to restore the adverse effects of orchidectomy on bone as measured by BMD, microstructural and biomechanical properties and bone remodeling markers.
本研究旨在评估甲状旁腺激素（PTH）（1-84）在去势诱导的雄性骨质疏松大鼠模型中的作用。
使用 6 月龄 Wistar 大鼠，分为以下几组：假手术（SHAM）组（模拟去势）、去势组（ORX）、去势+PTH1 组（ORX 并给予 10μg/Kg/d 的 PTH 1-84）和去势+PTH2 组（ORX 并给予 50μg/Kg/d 的 PTH 1-84），治疗从去势后 3 个月开始。
去势导致股骨和腰椎骨密度（BMD）降低，骨小梁和皮质微结构恶化，生物力学性能下降。两种剂量的 PTH（1-84）部分（低剂量）或完全（高剂量）恢复了 ORX 引起的变化。去势后血清 I 型胶原 C 端肽/抗酒石酸酸性磷酸酶 5b 同工酶（CTX/TRAP）吸收指数增加。骨钙素（骨 Gla 蛋白；BGP）水平不受去势影响。PTH（1-84）治疗对 CTX/TRAP 水平与 ORX 组相比没有任何变化。BGP 水平随 PTH 治疗而增加。
PTH（1-84）能够恢复去势对骨密度、微结构和生物力学性能以及骨重建标志物的不良影响。
