Bu S Y, Lucas E A, Franklin M, Marlow D, Brackett D J, Boldrin E A, Devareddy L, Arjmandi B H, Smith B J
Department of Nutritional Sciences, College of Human Environmental Science, Oklahoma State University, Stillwater, OK 74078, USA.
Osteoporos Int. 2007 Jul;18(7):931-42. doi: 10.1007/s00198-007-0335-y. Epub 2007 Feb 15.
Bone loss was confirmed after 90 days in 50 6-month-old male Sprague Dawley rats that were sham-operated or orchidectomized (ORX). In this study, we have shown that dried plum (DP) has potent effects on bone in terms of bone mass, microarchitecture, and strength in osteopenic male rats. Although these changes may be mediated through the suppression of bone resorption, the fact that the restoration in some of the bone structural and biomechanical parameter shares some similarities with parathyroid hormone (PTH) should not be overlooked. Further investigation is needed on a mechanistic level to clarify the influence of DP on bone metabolism.
This study was designed to investigate the extent to which DP reverses bone loss in osteopenic ORX rats and to compare its effects to PTH.
Fifty, 6-month-old male Sprague Dawley rats were sham-operated or ORX, and bone loss was confirmed after 90 days. The ORX groups were assigned to control (AIN-93M) diet, 25% DP diet, or PTH (80 microg/kg) for 90 days.
DP induced an 11% increase in vertebral and femoral BMD compared to ORX-controls. BMD in the PTH-treated group was increased by 20.7% (vertebra) and 17.9% (femur). Vertebral trabecular bone volume (BV/TV) and number were increased by DP and trabecular separation was decreased compared to controls, which were similar to PTH. Alterations in trabecular bone of the femur were similar to those in the vertebra, but DP did not restore BV/TV to the same extent. Cortical thickness was improved by DP and further enhanced by PTH. DP tended to decrease urinary deoxypyridinoline and calcium, but did not alter alkaline phosphatase or osteocalcin.
We conclude that though the degree of improvement was not equivalent to PTH with regard to all parameters, DP reverses bone loss due to ORX and the mechanisms should be further investigated.
在50只6月龄雄性斯普拉格-道利大鼠中，假手术组或去势组（ORX）在90天后证实出现骨质流失。在本研究中，我们已经表明，干李子（DP）对骨质减少的雄性大鼠的骨量、微结构和强度方面具有显著作用。尽管这些变化可能是通过抑制骨吸收介导的，但一些骨结构和生物力学参数的恢复与甲状旁腺激素（PTH）有一些相似之处这一事实不应被忽视。需要在机制层面进行进一步研究，以阐明DP对骨代谢的影响。
本研究旨在调查DP逆转骨质减少的去势大鼠骨质流失的程度，并将其效果与PTH进行比较。
50只6月龄雄性斯普拉格-道利大鼠接受假手术或去势手术，90天后证实出现骨质流失。去势组被分配到对照（AIN-93M）饮食、25% DP饮食或PTH（80微克/千克）组，持续90天。
与去势对照组相比，DP使椎骨和股骨骨密度增加了11%。PTH治疗组的骨密度分别增加了20.7%（椎骨）和17.9%（股骨）。与对照组相比，DP增加了椎骨小梁骨体积（BV/TV）和数量，减少了小梁间距，这与PTH相似。股骨小梁骨的变化与椎骨相似，但DP没有将BV/TV恢复到相同程度。DP改善了皮质厚度，PTH进一步增强了皮质厚度。DP倾向于降低尿脱氧吡啶啉和钙，但没有改变碱性磷酸酶或骨钙素。
我们得出结论，尽管在所有参数方面改善程度与PTH不等同，但DP可逆转去势导致的骨质流失，其机制应进一步研究。
