Bulygina E S, Galchenko V F, Govorukhina N I, Netrusov A I, Nikitin D I, Trotsenko Y A, Chumakov K M
Institute of Microbiology, Academy of Sciences, Moscow, USSR.
J Gen Microbiol. 1990 Mar;136(3):441-6. doi: 10.1099/00221287-136-3-441.
Nucleotide sequences of 5S ribosomal RNA (rRNA) isolated from 19 strains of Gram-negative methylotrophic bacteria were determined. Comparison of these sequences allowed construction of a tentative phylogenetic tree and showed that the bacteria analysed belong to the Proteobacteria and fell into several clusters, including obligate methanotrophs, obligate methylotrophs and several groups of facultative methylotrophs. Taxonomic relations between methylotrophic and non-methylotrophic bacteria are discussed, and the polyphyletic nature of methylotrophy as a taxonomic feature is highlighted.
测定了从19株革兰氏阴性甲基营养细菌中分离出的5S核糖体RNA（rRNA）的核苷酸序列。通过比较这些序列构建了一个初步的系统发育树，结果表明所分析的细菌属于变形菌门，并且分为几个簇，包括专性甲烷营养菌、专性甲基营养菌和几组兼性甲基营养菌。讨论了甲基营养细菌与非甲基营养细菌之间的分类关系，并强调了甲基营养作为一种分类特征的多系性质。
