Janvier M, Grimont P A
Unité des Entérobactéries, INSERM Unité 389, Institut Pasteur, Paris.
Res Microbiol. 1995 Sep;146(7):543-50. doi: 10.1016/0923-2508(96)80560-2.
The genus Methylophaga with two species, M. marina and M. thalassica, comprises halophilic methylotrophic bacteria. These organisms utilise C1 compounds through the ribulose monophosphate pathway and are unable to grow on methane. Nearly complete 16S rRNA sequences were obtained for both Methylophaga species by directly sequencing the amplified 16S rRNA gene. These sequences were compared with published 16S rRNA sequences of methylotrophic strains and a large number of marine bacterial strains including several members of the alpha, beta and gamma subclasses of Proteobacteria. Phylogenetic trees were inferred using both parsimony and distance matrix methods. Each topology was analysed by bootstrap. The genus Methylophaga was found to be clearly separated from other methylotrophic bacteria and formed a distinct branch within the gamma subclass of Proteobacteria.
嗜甲基菌属包含嗜盐甲基营养菌，有两个物种，即海洋嗜甲基菌和嗜甲基海杆菌。这些生物体通过磷酸戊糖途径利用C1化合物，无法在甲烷上生长。通过对扩增的16S rRNA基因进行直接测序，获得了两种嗜甲基菌属物种近乎完整的16S rRNA序列。将这些序列与已发表的甲基营养菌株以及大量海洋细菌菌株（包括变形菌门α、β和γ亚类的几个成员）的16S rRNA序列进行了比较。使用简约法和距离矩阵法推断系统发育树。通过自展法分析每种拓扑结构。发现嗜甲基菌属与其他甲基营养菌明显分开，并在变形菌门γ亚类中形成了一个独特的分支。
