Department of Obstetrics and Gynecology, Guangdong General Hospital and Guangdong Academy of Medical Sciences, Number 106 Zhongshan Road 2, Guangzhou 510080, China.
Taiwan J Obstet Gynecol. 2013 Jun;52(2):233-40. doi: 10.1016/j.tjog.2013.04.014.
Perimenopausal women are at high risk for pelvic organ prolapse (POP) and stress urinary incontinence (SUI) diseases. In the present study, the expression of VIP in the vaginal epithelium of 70 perimenopausal women was correlated with the severity of POP with or without SUI.
Seventy biopsy specimens from the anterior vaginal epithelium were obtained from postmenopausal patients. Immunohistochemical labeling for vasoactive intestinal peptide (VIP) and hematoxylin and eosin staining were performed. The VIP innervation was then compared between eight patient groups. Semiquantitative analysis of VIP protein by Western blotting was performed and compared between the eight patient groups.
The results of the immunohistochemical study showed that the intensity of VIP-immunoreactivity (VIP-ir) in the eight groups was as follows (in decreasing order): Control; POPI; POP II; POP II + SUI; POP III; POP IV and POP III + SUI; and POP IV + SUI. The intensity of VIP-ir was obviously weak and similar among the POP IV, POP III + SUI, and POP IV + SUI groups. This result was validated by the Western blotting analysis. The level of the VIP peptide also deceased in POP patients and was as follows (in decreasing order): Control; POPI; POP II and POP II + SUI; POP III and POP III + SUI; and POP IV and POP IV + SUI.
The present study found that reduced VIP innervation in the vaginal epithelium of the perimenopausal women was correlated with the severity of POP with or without SUI.
围绝经期女性患盆腔器官脱垂（POP）和压力性尿失禁（SUI）疾病的风险很高。本研究旨在探讨围绝经期女性阴道上皮中 VIP 的表达与伴有或不伴有 SUI 的 POP 严重程度的关系。
从绝经后患者中获得 70 个阴道前上皮活检标本。进行血管活性肠肽（VIP）免疫组织化学标记和苏木精-伊红染色。然后比较了 8 个患者组之间的 VIP 神经支配。通过 Western blot 对 VIP 蛋白进行半定量分析，并比较 8 个患者组之间的差异。
免疫组织化学研究结果显示，8 个组中 VIP 免疫反应性（VIP-ir）的强度如下（按强度降低的顺序排列）：对照组；POPI 组；POP II 组；POP II + SUI 组；POP III 组；POP IV 组和 POP III + SUI 组；POP IV + SUI 组。POP IV、POP III + SUI 和 POP IV + SUI 组中 VIP-ir 强度明显较弱且相似。Western blot 分析验证了这一结果。POP 患者 VIP 肽水平也降低，按降低顺序排列为：对照组；POPI 组；POP II 组和 POP II + SUI 组；POP III 组和 POP III + SUI 组；POP IV 组和 POP IV + SUI 组。
本研究发现，围绝经期女性阴道上皮中 VIP 神经支配减少与伴有或不伴有 SUI 的 POP 严重程度相关。
