接触镜护理用品的微生物污染及尼泊尔护理方案的遵守情况。

Microbial contamination of contact lens care accessories and compliance with care regimens in Nepal.

机构信息

College of Optometry, University of Houston, Houston, TX, USA.

Brien Holden Vision Institute, University of New South Wales, Sydney, NSW, Australia.

出版信息

Cont Lens Anterior Eye. 2014 Feb;37(1):2-10. doi: 10.1016/j.clae.2013.07.001. Epub 2013 Jul 31.

DOI:10.1016/j.clae.2013.07.001

PMID:23916116

Abstract

PURPOSE

To determine the rates of microbial contamination and identify contaminants associated with contact lens (CL) care accessories. To investigate self-reported compliance levels with care of CL accessories and its association with contamination level.

METHODS

A total of 46 CL cases and care solutions from asymptomatic soft contact lens wearers were collected. Samples from the CL cases and care solutions were cultured for microbiological identification and enumeration. A questionnaire regarding compliance to care and hygiene procedures were administered to each subject on their visit.

RESULTS

The percentage of microbial contamination for CL cases and care solutions was 50% and 10.9%, respectively. All the contaminants were bacterial. Staphylococcus aureus (37.0%, 17 of 46 cases) and Escherichia coli (8.7%, 4 of 46 cases) were the most common microorganisms detected in CL cases. In care solutions, S. aureus (8.7%, 4 of 46 samples), Pseudomonas aeruginosa (2.2%, 1 of 46 samples) were common contaminants. CL cases and care solutions that were used for <3 months were significantly less contaminated than those used for >3 months (10.9% versus 39.1%, p<0.05). Polyquad and Aldox based MPS solution showed no contamination. Subjects with medium or low compliance had highly significant rates of contaminations both in CL cases and solutions than for subjects with high compliance (p<0.05).

CONCLUSIONS

Nepalese Soft CL wearers are at high risk of developing ocular complications due to high CL case and solution contamination. Frequent replacement of CL cases and solutions, as well as good compliance is recommended to reduce risk of contamination.

摘要

目的

确定接触镜（CL）护理附件相关微生物污染率和污染物种类。调查 CL 护理附件的自我报告依从率及其与污染水平的关系。

方法

共采集 46 例无症状软镜佩戴者的 CL 及护理液样本。对 CL 样本和护理液样本进行微生物培养，以进行鉴定和计数。在每位受试者就诊时，对他们进行有关护理和卫生程序依从性的问卷调查。

结果

CL 及护理液的微生物污染率分别为 50%和 10.9%。所有污染物均为细菌。CL 中最常见的微生物为金黄色葡萄球菌（37.0%，46 例中有 17 例）和大肠杆菌（8.7%，46 例中有 4 例）。护理液中，金黄色葡萄球菌（8.7%，46 个样本中有 4 个）和铜绿假单胞菌（2.2%，46 个样本中有 1 个）是常见污染物。使用时间<3 个月的 CL 及护理液明显比使用时间>3 个月的污染程度低（10.9%比 39.1%，p<0.05）。基于聚六亚甲基双胍和多粘菌素 B 的 MPS 溶液无污染。中低依从性的受试者 CL 及护理液的污染率明显高于高依从性的受试者（p<0.05）。