Department of Biomedical Engineering, Faculty of Fundamental Problems of Technology, Wroclaw University of Science and Technology, 50-370 Wroclaw, Poland.
Research and Development Centre, Regional Specialist Hospital in Wroclaw, 73A H. M. Kamienskiego St., 51-124 Wroclaw, Poland.
Int J Mol Sci. 2024 Feb 24;25(5):2653. doi: 10.3390/ijms25052653.
The increase in bacterial resistance to antibiotics in recent years demands innovative strategies for the detection and combating of biofilms, which are notoriously resilient. Biofilms, particularly those on contact lenses, can lead to biofilm-related infections (e.g., conjunctivitis and keratitis), posing a significant risk to patients. Non-destructive and non-contact sensing techniques are essential in addressing this threat. Digital holographic tomography emerges as a promising solution. This allows for the 3D reconstruction of the refractive index distribution in biological samples, enabling label-free visualization and the quantitative analysis of biofilms. This tool provides insight into the dynamics of biofilm formation and maturation on the surface of transparent materials. Applying digital holographic tomography for biofilm examination has the potential to advance our ability to combat the antibiotic bacterial resistance crisis. A recent study focused on characterizing biofilm formation and maturation on six soft contact lens materials (three silicone hydrogels, three hydrogels), with a particular emphasis on and , both common culprits in ocular infections. The results revealed species- and time-dependent variations in the refractive indexes and volumes of biofilms, shedding light on cell dynamics, cell death, and contact lens material-related factors. The use of digital holographic tomography enables the quantitative analysis of biofilm dynamics, providing us with a better understanding and characterization of bacterial biofilms.
近年来，细菌对抗生素的耐药性不断增加，这就需要创新的策略来检测和对抗生物膜，因为生物膜具有很强的耐药性。生物膜，特别是隐形眼镜上的生物膜，会导致与生物膜相关的感染（例如结膜炎和角膜炎），这对患者构成了重大风险。非破坏性和非接触式传感技术在应对这一威胁方面至关重要。数字全息层析成像技术应运而生，成为一种很有前途的解决方案。该技术可以实现生物样本折射率分布的 3D 重建，实现无标记可视化和生物膜的定量分析。该工具可以深入了解透明材料表面生物膜形成和成熟的动态过程。将数字全息层析成像技术应用于生物膜检查有可能提高我们对抗抗生素细菌耐药性危机的能力。最近的一项研究侧重于研究六种软性隐形眼镜材料（三种硅水凝胶，三种水凝胶）上生物膜的形成和成熟，特别关注 和 ，它们都是眼部感染的常见罪魁祸首。结果表明，生物膜的折射率和体积存在物种和时间依赖性变化，这揭示了细胞动力学、细胞死亡以及与隐形眼镜材料相关的因素。数字全息层析成像技术的使用可以对生物膜动力学进行定量分析，使我们能够更好地理解和描述细菌生物膜。
