利用斑马鱼进行造血分析。
Assaying hematopoiesis using zebrafish.
机构信息
Stem Cell Program and Division of Hematology/Oncology, Children's Hospital Boston, Harvard Medical School, Boston, MA, USA.
出版信息
Blood Cells Mol Dis. 2013 Dec;51(4):271-6. doi: 10.1016/j.bcmd.2013.07.009. Epub 2013 Aug 2.
Abstract
The zebrafish has become a commonly used model for studying hematopoiesis as a result of its unique attributes. Zebrafish are highly suitable for large-scale genetic and chemical screens compared to other vertebrate systems. It is now possible to analyze hematopoietic lineages in zebrafish and validate cell function via transplantation assays. Here, we review advancements over the past decade in forward genetic screens, chemical screens, fluorescence-activated cell sorting analysis, and transplantation assays. Integrating these approaches enables new chemical and genetic screens that assay cell function within the hematopoietic system. Studies in zebrafish will continue to contribute and expand our knowledge about hematopoiesis, and develop novel treatments for clinical applications.
摘要
由于其独特的属性，斑马鱼已成为研究造血的常用模型。与其他脊椎动物系统相比，斑马鱼非常适合进行大规模的遗传和化学筛选。现在可以在斑马鱼中分析造血谱系，并通过移植实验验证细胞功能。在这里，我们回顾了过去十年中在正向遗传筛选、化学筛选、荧光激活细胞分选分析和移植实验方面的进展。整合这些方法可以进行新的化学和遗传筛选，以检测造血系统内的细胞功能。斑马鱼的研究将继续为我们对造血的认识做出贡献并扩展，并为临床应用开发新的治疗方法。
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