Key Laboratory of Animal Virology of Ministry of Agriculture and College of Life Sciences and Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education, Zhejiang University, Hangzhou 310058, People's Republic of China; College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, People's Republic of China.
Dev Comp Immunol. 2013 Dec;41(4):491-7. doi: 10.1016/j.dci.2013.07.015. Epub 2013 Aug 1.
Phagocytosis plays important roles in innate and adaptive immunity in animals. Some small G proteins are found to be related to phagocytosis. However, the Ran GTPase has not been intensively characterized in immunity. In this paper, the sequence analysis showed that the Ran was highly conserved in animals, suggesting that its function was preserved during animal evolution. The results showed that Ran was upregulated in S2 cells in response to DCV infection. It was further revealed that the antiviral phagocytosis could be mediated by Ran in S2 cells. By comparison with the early marker and late marker of phagosomes, the results showed that the Ran protein played an essential role at the early stage of phagocytosis or throughout the entire phagocytic process. Therefore our findings enlarged our limited knowledge about the phagocytosis regulation by small G proteins concerning to the nucleus.
吞噬作用在动物的先天免疫和适应性免疫中起着重要作用。一些小 G 蛋白被发现与吞噬作用有关。然而，Ran GTPase 在免疫方面的研究还不够深入。本文通过序列分析发现，Ran 在动物中高度保守，表明其功能在动物进化过程中得到了保留。结果表明，Ran 在 S2 细胞中受到 DCV 感染的诱导而上调。进一步的研究表明，Ran 可以在 S2 细胞中介导抗病毒吞噬作用。通过与吞噬体的早期和晚期标记物进行比较，结果表明 Ran 蛋白在吞噬作用的早期或整个吞噬过程中都发挥着重要作用。因此，我们的发现扩大了人们对小 G 蛋白调节吞噬作用的有限认识，涉及到细胞核。
