过氧化物酶体生物发生缺陷可防止载脂酵母中α-突触核蛋白与脂滴的结合。
A peroxisome biogenesis deficiency prevents the binding of alpha-synuclein to lipid droplets in lipid-loaded yeast.
机构信息
Department of Biochemistry and Molecular Biology, Louisiana State University Health Sciences Center, Shreveport, LA 71130, USA.
出版信息
Biochem Biophys Res Commun. 2013 Aug 23;438(2):452-6. doi: 10.1016/j.bbrc.2013.07.100. Epub 2013 Jul 31.
Abstract
Using a yeast model of Parkinson's disease, we found that alpha-synuclein (αS) binds to lipid droplets in lipid-loaded, wild-type yeast cells but not to lipid droplets in lipid-loaded, peroxisome-deficient cells (pex3Δ). Our analysis revealed that pex3Δ cells have both fewer lipid droplets and smaller lipid droplets than wild-type cells, and that the acyl chains of the phospholipids on the surface of the lipid droplets from pex3Δ cells are on average shorter (C16) than those (C18) on the surface of lipid droplets from wild-type cells. We propose that the shift to shorter (C18→C16) acyl chains contributes to the reduced binding of αS to lipid droplets in pex3Δ cells.
摘要
利用帕金森病的酵母模型，我们发现α-突触核蛋白（αS）与载脂的野生型酵母细胞中的脂滴结合，但不与载脂的过氧化物酶体缺陷型细胞（pex3Δ）中的脂滴结合。我们的分析表明，pex3Δ细胞中的脂滴数量少于野生型细胞，且pex3Δ细胞中的脂滴表面的磷脂酰基链的平均长度（C16）也短于野生型细胞（C18）。我们提出，向较短的（C18→C16）酰基链的转变有助于减少αS与pex3Δ细胞中脂滴的结合。
相似文献
1
A peroxisome biogenesis deficiency prevents the binding of alpha-synuclein to lipid droplets in lipid-loaded yeast.过氧化物酶体生物发生缺陷可防止载脂酵母中α-突触核蛋白与脂滴的结合。
Biochem Biophys Res Commun. 2013 Aug 23;438(2):452-6. doi: 10.1016/j.bbrc.2013.07.100. Epub 2013 Jul 31.
2
Peroxisome remnants in pex3delta cells and the requirement of Pex3p for interactions between the peroxisomal docking and translocation subcomplexes.pex3δ细胞中的过氧化物酶体残余物以及Pex3p在过氧化物酶体对接和易位子复合体相互作用中的需求。
Traffic. 2002 Aug;3(8):560-74. doi: 10.1034/j.1600-0854.2002.30806.x.
3
Yeast peroxisomes: How are they formed and how do they grow?酵母过氧化物酶体：它们是如何形成的，又是如何生长的？
Int J Biochem Cell Biol. 2018 Dec;105:24-34. doi: 10.1016/j.biocel.2018.09.019. Epub 2018 Sep 27.
4
Phosphorylation-dependent Pex11p and Fis1p interaction regulates peroxisome division.磷酸化依赖性 Pex11p 和 Fis1p 相互作用调节过氧化物酶体分裂。
Mol Biol Cell. 2012 Apr;23(7):1307-15. doi: 10.1091/mbc.E11-09-0782. Epub 2012 Feb 15.
5
The role of peroxisomes in xylose alcoholic fermentation in the engineered Saccharomyces cerevisiae.过氧化物酶体在工程化酿酒酵母木糖酒精发酵中的作用。
Cell Biol Int. 2020 Aug;44(8):1606-1615. doi: 10.1002/cbin.11353. Epub 2020 Apr 13.
6
The peroxin Pex34p functions with the Pex11 family of peroxisomal divisional proteins to regulate the peroxisome population in yeast.过氧化物酶体蛋白 Pex34p 与 Pex11 家族的过氧化物酶体分裂蛋白一起作用，以调节酵母中的过氧化物酶体群体。
Mol Biol Cell. 2011 May 15;22(10):1727-38. doi: 10.1091/mbc.E11-01-0084. Epub 2011 Mar 25.
7
Heat shock prevents alpha-synuclein-induced apoptosis in a yeast model of Parkinson's disease.热休克可预防帕金森病酵母模型中α-突触核蛋白诱导的细胞凋亡。
J Mol Biol. 2005 Sep 2;351(5):1081-100. doi: 10.1016/j.jmb.2005.06.060.
8
Peroxins Pex30 and Pex29 Dynamically Associate with Reticulons to Regulate Peroxisome Biogenesis from the Endoplasmic Reticulum.过氧化物酶体蛋白Pex30和Pex29与网织蛋白动态结合，以调节内质网来源的过氧化物酶体生物发生。
J Biol Chem. 2016 Jul 22;291(30):15408-27. doi: 10.1074/jbc.M116.728154. Epub 2016 Apr 29.
9
Pex11-related proteins in peroxisome dynamics: a role for the novel peroxin Pex27p in controlling peroxisome size and number in Saccharomyces cerevisiae.过氧化物酶体动态变化中的Pex11相关蛋白：新型过氧化物酶体蛋白Pex27p在控制酿酒酵母过氧化物酶体大小和数量中的作用
Mol Biol Cell. 2003 Oct;14(10):4089-102. doi: 10.1091/mbc.e03-03-0150. Epub 2003 May 18.
10
Alpha-synuclein selectively binds to anionic phospholipids embedded in liquid-disordered domains.α-突触核蛋白选择性地与嵌入液态无序结构域的阴离子磷脂结合。
J Mol Biol. 2008 Feb 1;375(5):1394-404. doi: 10.1016/j.jmb.2007.11.051. Epub 2007 Nov 22.
引用本文的文献
1
More than meets the eye in Parkinson's disease and other synucleinopathies: from proteinopathy to lipidopathy.在帕金森病和其他突触核蛋白病中，所见并非全部：从蛋白病变到脂类病变。
Acta Neuropathol. 2023 Sep;146(3):369-385. doi: 10.1007/s00401-023-02601-0. Epub 2023 Jul 8.
2
Peroxisomes of the Brain: Distribution, Functions, and Associated Diseases.脑内过氧化物酶体：分布、功能及相关疾病。
Neurotox Res. 2021 Jun;39(3):986-1006. doi: 10.1007/s12640-020-00323-9. Epub 2021 Jan 5.
3
Autophagy and Redox Homeostasis in Parkinson's: A Crucial Balancing Act.帕金森病中的自噬与氧化还原稳态：关键的平衡行为
Oxid Med Cell Longev. 2020 Nov 10;2020:8865611. doi: 10.1155/2020/8865611. eCollection 2020.
4
Peroxisome quality control and dysregulated lipid metabolism in neurodegenerative diseases.过氧化物酶体质量控制与神经退行性疾病中脂质代谢失调。
Exp Mol Med. 2020 Sep;52(9):1486-1495. doi: 10.1038/s12276-020-00503-9. Epub 2020 Sep 11.
5
Proteomics-Based Monitoring of Pathway Activity Reveals that Blocking Diacylglycerol Biosynthesis Rescues from Alpha-Synuclein Toxicity.基于蛋白质组学的通路活性监测显示，阻断二酰基甘油生物合成可挽救α-突触核蛋白毒性。
Cell Syst. 2019 Sep 25;9(3):309-320.e8. doi: 10.1016/j.cels.2019.07.010. Epub 2019 Sep 11.
6
Lipid Droplet and Peroxisome Biogenesis: Do They Go Hand-in-Hand?脂滴与过氧化物酶体生物发生：它们是携手并进的吗？
Front Cell Dev Biol. 2019 May 31;7:92. doi: 10.3389/fcell.2019.00092. eCollection 2019.
7
LRRK2 mediated Rab8a phosphorylation promotes lipid storage.LRRK2 介导的 Rab8a 磷酸化促进脂质储存。
Lipids Health Dis. 2018 Feb 27;17(1):34. doi: 10.1186/s12944-018-0684-x.
8
Peroxisomal Dysfunction in Age-Related Diseases.衰老相关疾病中的过氧化物酶体功能障碍
Trends Endocrinol Metab. 2017 Apr;28(4):297-308. doi: 10.1016/j.tem.2016.12.003. Epub 2017 Jan 4.
9
Peroxisomes are required for lipid metabolism and muscle function in Drosophila melanogaster.过氧化物酶体对于黑腹果蝇的脂质代谢和肌肉功能是必需的。
PLoS One. 2014 Jun 19;9(6):e100213. doi: 10.1371/journal.pone.0100213. eCollection 2014.
本文引用的文献
1
Pathological α-synuclein transmission initiates Parkinson-like neurodegeneration in nontransgenic mice.病理性α-突触核蛋白的传递会在非转基因小鼠中引发类似帕金森病的神经退行性变。
Science. 2012 Nov 16;338(6109):949-53. doi: 10.1126/science.1227157.
2
α-Synuclein disrupts stress signaling by inhibiting polo-like kinase Cdc5/Plk2.α-突触核蛋白通过抑制 Polo 样激酶 Cdc5/Plk2 破坏应激信号。
Proc Natl Acad Sci U S A. 2012 Oct 2;109(40):16119-24. doi: 10.1073/pnas.1206286109. Epub 2012 Sep 17.
3
α-Synuclein posttranslational modification and alternative splicing as a trigger for neurodegeneration.α-突触核蛋白的翻译后修饰和可变剪接作为神经退行性变的触发因素。
Mol Neurobiol. 2013 Apr;47(2):509-24. doi: 10.1007/s12035-012-8330-5. Epub 2012 Aug 25.
4
Peroxisome biogenesis disorders: molecular basis for impaired peroxisomal membrane assembly: in metabolic functions and biogenesis of peroxisomes in health and disease.过氧化物酶体生物发生障碍：过氧化物酶体膜组装受损的分子基础：健康与疾病中过氧化物酶体的代谢功能和生物发生
Biochim Biophys Acta. 2012 Sep;1822(9):1337-42. doi: 10.1016/j.bbadis.2012.06.004. Epub 2012 Jun 13.
5
Membrane curvature sensing by amphipathic helices: a single liposome study using α-synuclein and annexin B12.两亲性螺旋通过膜曲率感应：使用α-突触核蛋白和膜联蛋白 B12 的单个脂质体研究。
J Biol Chem. 2011 Dec 9;286(49):42603-42614. doi: 10.1074/jbc.M111.271130. Epub 2011 Sep 27.
6
Specificity and kinetics of alpha-synuclein binding to model membranes determined with fluorescent excited state intramolecular proton transfer (ESIPT) probe.用荧光激发态分子内质子转移（ESIPT）探针测定α-突触核蛋白与模型膜的特异性和动力学。
J Biol Chem. 2011 Apr 15;286(15):13023-32. doi: 10.1074/jbc.M110.204776. Epub 2011 Feb 17.
7
Alpha-synuclein promotes SNARE-complex assembly in vivo and in vitro.α-突触核蛋白在体内和体外促进 SNARE 复合物的组装。
Science. 2010 Sep 24;329(5999):1663-7. doi: 10.1126/science.1195227. Epub 2010 Aug 26.
8
19F NMR studies of α-synuclein-membrane interactions.19F NMR 研究α-突触核蛋白与膜的相互作用。
Protein Sci. 2010 Sep;19(9):1686-91. doi: 10.1002/pro.449.
9
Molecular mechanisms of alpha-synuclein neurodegeneration.α-突触核蛋白神经变性的分子机制
Biochim Biophys Acta. 2009 Jul;1792(7):616-24. doi: 10.1016/j.bbadis.2008.09.013. Epub 2008 Oct 9.
10
YGR198w (YPP1) targets A30P alpha-synuclein to the vacuole for degradation.YGR198w（YPP1）将A30Pα-突触核蛋白靶向液泡进行降解。
J Cell Biol. 2007 Jun 18;177(6):1091-104. doi: 10.1083/jcb.200610071.
Zotero 插件