Department of Veterinary Diagnostic Imaging, School of Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan.
Exp Parasitol. 2013 Oct;135(2):320-4. doi: 10.1016/j.exppara.2013.07.015. Epub 2013 Jul 31.
We herein describe the establishment of single hepatic lesions of Echinococcus multilocularis in rats. A 3mm incision was made on the liver with a surgical knife, and one small round vesicle of E. multilocularis (between 1 × 1 mm and <2 × 2 mm in diameter) was transplanted into the incision and covered with absorbable hemostat gauze. The presence and growth of the transplanted vesicle was monitored for 12 weeks using magnetic resonance imaging (MRI). Hepatic lesions, the metacestode of this parasite were confirmed in 12 of 17 infected rats (70.6%) by MRI and macroscopic examinations. The average size of the metacestodes with brood capsules at 12 weeks after the experimental transplantation of a single vesicle was 6.1 ± 2.5 mm × 4.4 ± 1.5mm. The smallest size of the metacestodes detected by MRI was approximately 3 × 3 mm. This new approach of establishing single hepatic metacestodes of E. multilocularis in experimental animals is expected to be useful for analyzing the immune-pathological mechanisms of hepatic AE.
我们在此描述了在大鼠中建立细粒棘球蚴（Echinococcus multilocularis）单发性肝病变的方法。用手术刀在肝脏上做一个 3mm 的切口，将一个小的圆形细粒棘球蚴囊泡（直径在 1×1mm 至<2×2mm 之间）移植到切口中，并覆盖可吸收止血纱布。使用磁共振成像（MRI）监测移植囊泡的存在和生长情况，共持续 12 周。通过 MRI 和大体检查，在 17 只感染大鼠中，有 12 只（70.6%）证实存在肝病变，即该寄生虫的中绦期幼虫。在单个囊泡实验性移植 12 周后，带囊壳的中绦期幼虫的平均大小为 6.1±2.5mm×4.4±1.5mm。通过 MRI 检测到的最小中绦期幼虫大小约为 3×3mm。这种在实验动物中建立细粒棘球蚴单发性肝病变的新方法，有望用于分析肝泡型包虫病的免疫病理学机制。
