Centre of Inflammation and Metabolism, August Krogh Centre, August Krogh Building, Department of Biology, University of Copenhagen, Copenhagen, Denmark.
Exp Gerontol. 2013 Nov;48(11):1274-84. doi: 10.1016/j.exger.2013.07.015. Epub 2013 Aug 3.
BACKGROUND/AIM: Age-related metabolic diseases are often associated with low-grade inflammation. The aim of the present study was to investigate the role of the transcriptional co-activator PGC-1α in the potential beneficial effects of exercise training and/or resveratrol in the prevention of age-associated low-grade inflammation. To address this, a long-term voluntary exercise training and resveratrol supplementation study was conducted.
Three month old whole body PGC-1α KO and WT mice were randomly assigned to four groups: untrained chow-fed, untrained chow-fed supplemented with resveratrol, chow-fed voluntarily exercise trained and chow-fed supplemented with resveratrol and voluntarily exercise trained. The intervention lasted 12 months and three month old untrained chow-fed mice served as young controls.
Voluntary exercise training prevented an age-associated increase (p<0.05) in systemic IL-6 and adiposity in WT mice. PGC-1α expression was required for a training-induced prevention of an age-associated increase (p<0.05) in skeletal muscle TNFα protein. Independently of PGC-1α, both exercise training and resveratrol prevented an age-associated increase (p<0.05) in skeletal muscle protein carbonylation.
The present findings highlight that exercise training is a more effective intervention than resveratrol supplementation in reducing age-associated inflammation and that PGC-1α in part is required for the exercise training-induced anti-inflammatory effects.
背景/目的：与年龄相关的代谢性疾病通常与低度炎症有关。本研究旨在探讨转录共激活因子 PGC-1α 在运动训练和/或白藜芦醇预防与年龄相关的低度炎症中的潜在有益作用中的作用。为了解决这个问题，进行了一项长期的自愿运动训练和白藜芦醇补充研究。
3 个月大的全身 PGC-1α KO 和 WT 小鼠被随机分为四组：未训练的标准饮食喂养组、未训练的标准饮食喂养加白藜芦醇补充组、标准饮食喂养的自愿运动训练组和标准饮食喂养加白藜芦醇补充的自愿运动训练组。干预持续 12 个月，3 个月大的未训练的标准饮食喂养的小鼠作为年轻对照组。
自愿运动训练可预防 WT 小鼠与年龄相关的全身 IL-6 和肥胖增加（p<0.05）。PGC-1α 的表达是训练诱导的预防骨骼肌 TNFα 蛋白与年龄相关的增加所必需的（p<0.05）。独立于 PGC-1α，运动训练和白藜芦醇均可预防与年龄相关的骨骼肌蛋白羰基化增加（p<0.05）。
本研究结果强调，运动训练是一种比白藜芦醇补充更有效的干预措施，可降低与年龄相关的炎症，而 PGC-1α 在一定程度上是运动训练诱导的抗炎作用所必需的。
