Freitas Patrícia Franchi, Borges Marisa Essenfelder, Ortolani-Machado Claudia Feijó
Laboratório de Biologia do Desenvolvimento, Departamento de Biologia Celular, Universidade Federal do Paraná, Centro Politécnico, Curitiba 81531-990, Paraná, Brazil.
Faculdades Integradas do Brasil, Rua Konrad Adenauer, 442, Curitiba 82820-540, Paraná, Brazil.
Acta Histochem. 2014 Jan;116(1):138-47. doi: 10.1016/j.acthis.2013.06.006. Epub 2013 Aug 3.
Melanoblasts are positively stimulated to migrate in the dorsolateral pathway of the avian embryo by ephrins, but are inhibited by PNA-binding glycoconjugates. We analyzed the potential role of these molecules in the Japanese Silky fowl, which displays intense internal pigmentation. The distribution of ephrin ligands was analyzed using Eph receptor-human Fc fusion proteins. Glycoconjugates were labeled using PNA-FITC. In Japanese Silky embryos, ventral areas, including the anterior- and posterior-half somites, expressed ephrin-B1 in a pattern that correlates with the atypical migratory pathways taken by Japanese Silky melanoblasts. White Leghorn embryos displayed little to no ephrin-Bs in the ventral paths. Conversely, PNA-binding barrier tissues, proposed to prevent melanoblasts from migrating ventrally in White Leghorn, are missing or have significant gaps in Japanese Silky embryos. Thus, studies of a naturally occurring pigmentation mutant confirm that a combination of cues regulates melanoblast migration in the chick embryo.
黑素母细胞受到ephrins的正向刺激，在禽类胚胎的背外侧途径中迁移，但受到与PNA结合的糖缀合物的抑制。我们分析了这些分子在具有强烈内部色素沉着的日本丝羽乌骨鸡中的潜在作用。使用Eph受体-人Fc融合蛋白分析ephrin配体的分布。使用PNA-FITC标记糖缀合物。在日本丝羽乌骨鸡胚胎中，包括前半体节和后半体节在内的腹侧区域以与日本丝羽乌骨鸡黑素母细胞所采用的非典型迁移途径相关的模式表达ephrin-B1。白来航鸡胚胎在腹侧路径中几乎不表达或不表达ephrin-Bs。相反，在白来航鸡中被认为可阻止黑素母细胞向腹侧迁移的与PNA结合的屏障组织，在日本丝羽乌骨鸡胚胎中缺失或有明显间隙。因此，对一种天然存在的色素沉着突变体的研究证实，多种信号的组合调节鸡胚中黑素母细胞的迁移。
