National Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics, Breeding, and Reproduction of the Ministry of Agriculture, China Agricultural University, Beijing 100193, China.
College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
Sci Rep. 2017 Apr 13;7:45959. doi: 10.1038/srep45959.
Hyperpigmentation in Silky Fowl (SF) results in aberrant immune cell development. However, how melanocytes regulate B-cell proliferation in the bursa of Fabricius (BF) is unclear. To resolve this conundrum, we collected BFs from three-week-old SF and White Leghorn (WL) female chickens for RNA sequencing. The BF development was relatively weaker in SF than in WL. The transcriptome analyses identified 4848 differentially expressed genes, 326 long noncoding RNAs (lncRNAs), and 67 microRNAs in the BF of SF. The genes associated with melanogenesis was significantly higher, but that of the genes associated with the cytokine-cytokine receptor interactions and JAK-STAT signalling pathway was significantly lower in SF than in WL. Crucial biological processes, such as the receptor activity, cell communication, and cellular responses to stimuli, were clustered in SF. The predicted target lncRNAs genes were mainly associated with cell proliferation pathways such as JAK-STAT, WNT, MAPK, and Notch signalling pathways. Except for the above pathways, the target microRNA genes were related to the metabolism, melanogenesis, autophagy, and NOD-like and Toll-like receptor signalling pathways. The lncRNAs and microRNAs were predicted to regulate the JAK2, STAT3, and IL-15 genes. Thus, B-cell development in the BF of SF might be regulated and affected by noncoding RNAs.
丝羽乌骨鸡（SF）的色素沉着导致免疫细胞发育异常。然而，黑素细胞如何调节法氏囊（BF）中的 B 细胞增殖尚不清楚。为了解决这个难题，我们从 3 周龄的 SF 和白来航（WL）雌性鸡中收集 BF 进行 RNA 测序。SF 中的 BF 发育相对较弱。转录组分析在 SF 的 BF 中鉴定出 4848 个差异表达基因、326 个长非编码 RNA（lncRNA）和 67 个 microRNA。SF 中的与黑色素生成相关的基因显着升高，但与细胞因子-细胞因子受体相互作用和 JAK-STAT 信号通路相关的基因显着降低。关键的生物学过程，如受体活性、细胞通讯和细胞对刺激的反应，在 SF 中聚类。预测的靶 lncRNA 基因主要与 JAK-STAT、WNT、MAPK 和 Notch 信号通路等细胞增殖途径相关。除了上述途径外，靶 microRNA 基因还与代谢、黑色素生成、自噬以及 NOD 样和 Toll 样受体信号通路有关。lncRNA 和 microRNA 被预测可调节 JAK2、STAT3 和 IL-15 基因。因此，SF 的 BF 中的 B 细胞发育可能受到非编码 RNA 的调节和影响。
