International Postgraduate Program in Environmental Management, Graduate School, Chulalongkorn University, Bangkok 10330, Thailand; Center of Excellence on Hazardous Substance Management (HSM), Bangkok 10330, Thailand.
Department of Microbiology, Faculty of Science, Prince of Songkla University, Hat Yai Campus, Hat Yai, Songkhla 90110, Thailand.
Bioresour Technol. 2013 Oct;146:101-108. doi: 10.1016/j.biortech.2013.07.051. Epub 2013 Jul 20.
Biochemical methane potential (BMP) assay was conducted at 35 °C to evaluate the effects of inoculum to substrate ratio (ISR) and substrate mix ratio between para-grass and pig manure co-digesting using different inocula. Rubber latex digester (RLD) inoculum showed higher methanogenic activity (41.4 mL CH4/gVS) than pig farm digester (PFD) inoculum (37.3 mL CH4/gVS). However, the maximum methane yields, occurred at the highest para-grass mix ratio (G), were 369.6, 437.6, 465.9 and 442.6 mL CH4/gTSadded for RLD inoculum, versus 332.4, 475.0, 519.5 and 521.9 mL/gTSadded for PFD inoculum at ISR 1, 2, 3, and 4, respectively. HPr, HBu and HVa appeared at higher G, corresponding to substrate's higher biodegradability. Response surface indicated that higher ISR and G had a significantly positive impact on methane yield. It suggested the use of higher ISR, i.e. 3 or 4, for BMP assay of these co-substrates. Dominant species of fermentative bacteria in each inoculum was tested by DGGE.
采用生化甲烷潜能（BMP）测定法在 35°C 下进行，以评估接种物与底物比（ISR）和百喜草与猪粪共消化底物混合物比例对不同接种物的影响。胶乳消化器（RLD）接种物的产甲烷活性（41.4 mL CH4/gVS）高于猪场消化器（PFD）接种物（37.3 mL CH4/gVS）。然而，最大甲烷产量出现在百喜草混合比例最高（G）时，RLD 接种物的最大甲烷产量分别为 369.6、437.6、465.9 和 442.6 mL CH4/gTSadded，而 PFD 接种物的最大甲烷产量分别为 332.4、475.0、519.5 和 521.9 mL/gTSadded，ISR 分别为 1、2、3 和 4。HPr、HBu 和 HVa 出现在更高的 G 时，对应于底物更高的生物降解性。响应面表明，较高的 ISR 和 G 对甲烷产量有显著的积极影响。这表明在这些共底物的 BMP 测定中使用较高的 ISR，即 3 或 4。通过 DGGE 测试了每种接种物中发酵细菌的优势种。
