Centre for Translational Bone, Joint and Soft Tissue Research, Medical Faculty and University Hospital, Technische Universität Dresden, Dresden, Germany.
Acta Biomater. 2013 Dec;9(12):9547-57. doi: 10.1016/j.actbio.2013.07.027. Epub 2013 Jul 31.
In the present study, the in vitro effects of novel strontium-modified calcium phosphate bone cements (SrCPCs), prepared using two different approaches on human-bone-marrow-derived mesenchymal stem cells (hMSCs), were evaluated. Strontium ions, known to stimulate bone formation and therefore already used in systemic osteoporosis therapy, were incorporated into a hydroxyapatite-forming calcium phosphate bone cement via two simple approaches: incorporation of strontium carbonate crystals and substitution of Ca(2+) by Sr(2+) ions during cement setting. All modified cements released 0.03-0.07 mM Sr(2+) under in vitro conditions, concentrations that were shown not to impair the proliferation or osteogenic differentiation of hMSCs. Furthermore, strontium modification led to a reduced medium acidification and Ca(2+) depletion in comparison to the standard calcium phosphate cement. In indirect and direct cell culture experiments with the novel SrCPCs significantly enhanced cell proliferation and differentiation were observed. In conclusion, the SrCPCs described here could be beneficial for the local treatment of defects, especially in the osteoporotic bone.
在本研究中，评估了两种不同方法制备的新型锶改性磷酸钙骨水泥（SrCPC）对人骨髓间充质干细胞（hMSCs）的体外影响。锶离子已知能刺激骨形成，因此已用于系统性骨质疏松症治疗，通过两种简单的方法将其掺入到羟磷灰石形成的磷酸钙骨水泥中：掺入碳酸锶晶体和在水泥凝固过程中用 Sr(2+) 离子取代 Ca(2+)。所有改性水泥在体外条件下释放 0.03-0.07mM Sr(2+)，其浓度不会损害 hMSCs 的增殖或成骨分化。此外，与标准磷酸钙水泥相比，锶改性导致培养基酸化和 Ca(2+) 耗竭减少。在新型 SrCPC 的间接和直接细胞培养实验中，观察到细胞增殖和分化明显增强。总之，这里描述的 SrCPC 可能有益于局部治疗缺陷，特别是在骨质疏松性骨中。
