SbcCD 介导的大肠埃希菌共价拓扑异构酶-DNA 复合物的加工。
SbcCD-mediated processing of covalent gyrase-DNA complex in Escherichia coli.
机构信息
Department of Biochemistry & Molecular Biology, New York Medical College, Valhalla, New York, USA.
出版信息
Antimicrob Agents Chemother. 2013 Oct;57(10):5116-9. doi: 10.1128/AAC.00130-13. Epub 2013 Aug 5.
Abstract
Quinolones trap the covalent gyrase-DNA complex in Escherichia coli, leading to cell death. Processing activities for trapped covalent complex have not been characterized. A mutant strain lacking SbcCD nuclease activity was examined for both accumulation of gyrase-DNA complex and viability after quinolone treatment. Higher complex levels were found in ΔsbcCD cells than in wild-type cells after incubation with nalidixic acid and ciprofloxacin. However, SbcCD activity protected cells against the bactericidal action of nalidixic acid but not ciprofloxacin.
摘要
喹诺酮类药物会捕获大肠埃希菌中的共价拓扑异构酶-DNA 复合物，从而导致细胞死亡。目前尚未对被捕获的共价复合物的加工活性进行表征。我们研究了缺乏 SbcCD 核酸内切酶活性的突变菌株在喹诺酮类药物处理后的共价拓扑异构酶-DNA 复合物积累和存活情况。与野生型细胞相比，在用萘啶酸和环丙沙星孵育后，Δ sbcCD 细胞中的复合物水平更高。然而，SbcCD 活性可保护细胞免受萘啶酸的杀菌作用，但不能保护细胞免受环丙沙星的杀菌作用。
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