Department of Obstetrics and Gynecology; Southwest Hospital; Third Military Medical University; Chongqing, P.R. China.
Cancer Biol Ther. 2013 Oct 1;14(10):937-48. doi: 10.4161/cbt.25940. Epub 2013 Aug 5.
Endoglin (CD105, END) is upregulated in proliferating endothelial cells, suggesting potential therapeutic properties. However, it is not clear whether endoglin mediates an enhanced proliferative rate or may be upregulated as part of a negative feedback loop. To gain insights into context-dependent and cell type-dependent regulatory effects of endoglin, we studied its role properties in human ovarian carcinoma-derived endothelial cells (ODMECs). We isolated and cultured primary ODMECs from epithelial ovarian carcinoma tissue. ODMECs had higher expression of endoglin and VEGFR-2, and also exhibited enhanced spontaneous formation of vessel-like structures in vitro. Transfection of siRNA targeting endoglin in ODMECs cells resulted in the reduction of the proliferation and tube formation. These results indicate that a subset of ODMECs display abnormal angiogenic properties and this phenotype was blocked by decreasing endoglin levels, suggesting endoglin is essential for stimulating angiogenesis, and targeting it may be an attractive approach to anti-angiogenesis therapy for ovarian carcinoma.
内皮糖蛋白（CD105，END）在增殖的内皮细胞中上调，提示其具有潜在的治疗特性。然而，目前尚不清楚内皮糖蛋白是否介导了增强的增殖率，或者是否作为负反馈回路的一部分而上调。为了深入了解内皮糖蛋白在依赖于上下文和依赖于细胞类型的调节作用，我们研究了其在人卵巢癌衍生的内皮细胞（ODMECs）中的作用特性。我们从上皮性卵巢癌组织中分离和培养了原代 ODMECs。ODMECs 中内皮糖蛋白和 VEGFR-2 的表达更高，并且在体外也表现出增强的自发性血管样结构形成。在 ODMECs 细胞中转染内皮糖蛋白靶向 siRNA 可导致增殖和管形成减少。这些结果表明，一部分 ODMECs 表现出异常的血管生成特性，这种表型可通过降低内皮糖蛋白水平而阻断，提示内皮糖蛋白对刺激血管生成是必需的，针对其可能是治疗卵巢癌抗血管生成的一种有吸引力的方法。
