Environmental Risk and Health unit, VITO (Flemish Institute for Technological Research), Boeretang 200, B-2400 Mol, Belgium.
Environ Int. 2013 Sep;59:418-24. doi: 10.1016/j.envint.2013.07.007. Epub 2013 Aug 3.
DNA methylation changes are potential pathways of environmentally induced health effects. We investigated whether exposure to ambient concentrations of NO2, PM10, PM2.5 and O3 and traffic parameters were associated with global DNA methylation in blood of healthy adults.
48 non-smoking adults (25 males) with a median age of 39years were sampled in winter and summer. Global DNA methylation in whole blood (% 5-methyl-2'-deoxycytidine, %5mdC) was analyzed with HPLC. Exposure to air pollutants at the home address was assessed using interpolated NO2, PM10, PM2.5 and O3 concentrations for various exposure windows (60- to 1-day moving average exposures and yearly averages) and GIS-based traffic parameters. Associations between pollutants and %5mdC were tested with multiple mixed effects regression models.
Average %5mdC (SD) was 4.30 (0.08) in winter and 4.29 (0.08) in summer. Men had higher %5mdC compared to women both in winter (4.32 vs. 4.26) and summer (4.31 vs. 4.27). When winter and summer data were analyzed together, various NO2, PM10 and PM2.5 moving average exposures were associated with changes in %5mdC (95% CI) ranging from -0.04 (-0.09 to 0.00) to -0.14 (-0.28 to 0.00) per IQR increase in pollutant. NO2, PM10, PM2.5 and O3 moving average exposures were associated with decreased %5mdC (95% CI) varying between -0.01 (-0.03 to 0.00) and -0.17 (-0.27 to -0.06) per IQR increase in pollutant in summer but not in winter.
Decreased global DNA methylation in whole blood was associated with exposure to NO2, PM10, PM2.5 and O3 at the home addresses of non- adults. Most effects were observed for the 5- to 30-day moving average exposures.
DNA 甲基化变化是环境引起健康影响的潜在途径。我们研究了大气污染物（NO2、PM10、PM2.5 和 O3）浓度以及交通参数是否与健康成年人血液中的全基因组 DNA 甲基化有关。
在冬季和夏季，共采集了 48 名非吸烟成年人（25 名男性）的样本，中位年龄为 39 岁。采用高效液相色谱法（HPLC）分析全血中的全基因组 DNA 甲基化（5-甲基-2'-脱氧胞苷，%5mdC）。通过 GIS 交通参数和家庭住址的污染物浓度内插值，评估不同暴露窗口（60-1 天移动平均暴露和年平均浓度）的空气污染物暴露情况。采用多元混合效应回归模型检验污染物与%5mdC 之间的相关性。
冬季和夏季的平均%5mdC（标准差）分别为 4.30（0.08）和 4.29（0.08）。男性的%5mdC 高于女性，冬季为 4.32（4.26），夏季为 4.31（4.27）。当将冬季和夏季的数据合并分析时，各种 NO2、PM10 和 PM2.5 移动平均暴露与%5mdC 的变化相关，污染物每增加一个 IQR，%5mdC 的变化范围为-0.04（-0.09 至 0.00）至-0.14（-0.28 至 0.00）。NO2、PM10、PM2.5 和 O3 的移动平均暴露与全血中%5mdC 的降低相关（95%CI），在夏季，污染物每增加一个 IQR，%5mdC 的变化范围为-0.01（-0.03 至 0.00）至-0.17（-0.27 至-0.06），而冬季则没有这种相关性。
非成年人家庭住址的大气污染物（NO2、PM10、PM2.5 和 O3）暴露与全血中全基因组 DNA 甲基化的降低有关。大多数影响出现在 5-30 天的移动平均暴露中。
