Chen Lu-Min, Wang Weu, Lee Jen-Chih, Chiu Feng-Hsiang, Wu Chun-Te, Tai Cheng-Jeng, Wang Chien-Kai, Tai Chen-Jei, Huang Ming-Te, Chang Yu-Jia
Department of Obstetrics and Gynecology, Suzhou BenQ Medical Center, Nanjing Medical University, Suzhou, People's Republic of China.
Tumour Biol. 2013 Dec;34(6):3743-51. doi: 10.1007/s13277-013-0958-x. Epub 2013 Aug 6.
Thrombomodulin (TM), a natural anticoagulation factor, maintains circulation homeostasis in endothelial cells. TM has additional roles in modulating inflammation, thrombosis, and carcinogenesis. However, there is little information on the role of TM in the progression and metastasis of ovarian cancer. RNA silencing and cDNA expression vectors were used to manipulate target gene expression in ovarian cancer cells. Cell growth and migration were evaluated by an MTT assay, a wound-healing migration assay, a transwell migration assay, and a biosensor system. In this study, we found that TM silencing may enhance the growth rate of cells. The migratory ability of ovarian cancer cells was enhanced dramatically after TM silencing. TM overexpression in ovarian cells suppressed the proliferation and migration capability. Furthermore, we found that skov-3 cells treated with TM shRNA expressed high levels of fibronectin and vimentin and that the expression of these markers correlated positively with their migratory ability. Our results demonstrate that TM expression may regulate cell growth and migration in ovarian cancer cells. This finding suggests that TM may be a novel prognostic and therapeutic target for ovarian cancer.
血栓调节蛋白（TM）是一种天然抗凝因子，可维持内皮细胞中的循环稳态。TM在调节炎症、血栓形成和致癌作用方面还具有其他作用。然而，关于TM在卵巢癌进展和转移中的作用的信息很少。RNA沉默和cDNA表达载体被用于操纵卵巢癌细胞中的靶基因表达。通过MTT法、伤口愈合迁移试验、Transwell迁移试验和生物传感器系统评估细胞生长和迁移。在本研究中，我们发现TM沉默可能会提高细胞的生长速率。TM沉默后，卵巢癌细胞的迁移能力显著增强。卵巢细胞中TM的过表达抑制了增殖和迁移能力。此外，我们发现用TM shRNA处理的skov-3细胞表达高水平的纤连蛋白和波形蛋白，并且这些标志物的表达与其迁移能力呈正相关。我们的结果表明，TM表达可能调节卵巢癌细胞的生长和迁移。这一发现表明，TM可能是卵巢癌一种新的预后和治疗靶点。
