Estephan Zaki G, Schlenoff Joseph B
Florida State University, Tallahassee, FL, USA.
Methods Mol Biol. 2013;1025:201-5. doi: 10.1007/978-1-62703-462-3_15.
Passivating surfaces against protein adsorption is important for many biotechnological applications. Current approaches have been exploiting the use of zwitterions instead of poly(ethylene glycol) (PEG). Commonly used zwitterions are polymeric and are grafted onto surfaces using specialized polymerization techniques. Here we describe the synthesis of a monomeric zwitterion siloxane and its covalent attachment to silica surfaces (nanoparticle and planar) in a one-pot one-step aqueous method requiring no catalyst.
使表面钝化以防止蛋白质吸附对于许多生物技术应用来说都很重要。目前的方法一直在利用两性离子而非聚乙二醇（PEG）。常用的两性离子是聚合物，通过专门的聚合技术接枝到表面上。在此，我们描述了一种单体两性离子硅氧烷的合成方法，以及在无需催化剂的一锅一步水相法中，将其共价连接到二氧化硅表面（纳米颗粒和平面）的方法。
