疏水性药物在金纳米颗粒上的非共价细胞内药物递送。

Noncovalent intracellular drug delivery of hydrophobic drugs on Au NPs.

作者信息

Doane Tennyson, Burda Clemens

机构信息

Case Western Reserve University, Cleveland, OH, USA.

出版信息

Methods Mol Biol. 2013;1025:251-60. doi: 10.1007/978-1-62703-462-3_19.

DOI:10.1007/978-1-62703-462-3_19

PMID:23918343

Abstract

The successful delivery of hydrophobic drugs to cellular targets continues to present challenges to the pharmaceutical industry. The advances made by nanotechnology have generated new avenues for selectively loading, delivering, and targeting these drugs to their biological targets without compromising efficacy. Here, we describe how gold nanoparticles (Au NPs) functionalized with polyethylene glycol (PEG) can be evaluated for the delivery of hydrophobic drugs in aqueous systems. Specifically, we describe Au NP synthesis, ligand exchange, and delivery evaluation at-the-bench for screening of potential drug candidates.

摘要

将疏水性药物成功递送至细胞靶点仍然是制药行业面临的挑战。纳米技术的进步为选择性加载、递送这些药物并将其靶向至生物靶点开辟了新途径，同时不影响疗效。在此，我们描述了如何评估用聚乙二醇（PEG）功能化的金纳米颗粒（Au NPs）在水性体系中递送疏水性药物的能力。具体而言，我们描述了用于筛选潜在候选药物的Au NP合成、配体交换及台式递送评估。