通过聚乙二醇化在生理条件下稳定金纳米颗粒生物共轭物。

Stabilizing gold nanoparticle bioconjugates in physiological conditions by PEGylation.

作者信息

Comenge Joan, Puntes Víctor F

机构信息

Catalan Institute of Nanotechnology, Universitat Autònoma de Barcelona, Barcelona, Spain.

出版信息

Methods Mol Biol. 2013;1025:281-9. doi: 10.1007/978-1-62703-462-3_22.

DOI:10.1007/978-1-62703-462-3_22

PMID:23918346

Abstract

Stability of gold nanoparticles (AuNPs) is often compromised in physiological conditions. The loss of colloidal stability might lead to undesired biological responses for drug delivery nanosystems. Here a methodology to confer additional stability to the AuNPs by the addition of PEG is presented. Also, protocols to prepare and characterize the composition and conformation of mixed layers with PEG and alkanethiols are described here. Finally, methods to assay the stability of the link between the NP conjugates and a model drug are shown.

摘要

金纳米颗粒（AuNPs）的稳定性在生理条件下常常受到影响。胶体稳定性的丧失可能会导致药物递送纳米系统产生不良的生物反应。本文提出了一种通过添加聚乙二醇（PEG）赋予金纳米颗粒额外稳定性的方法。此外，还描述了制备和表征PEG与链烷硫醇混合层的组成和构象的方案。最后，展示了测定纳米颗粒缀合物与模型药物之间连接稳定性的方法。