用于基因传递载体的碳纳米管修饰

Modification of carbon nanotubes for gene delivery vectors.

作者信息

Ramos-Perez Victor, Cifuentes Anna, Coronas Núria, de Pablo Ana, Borrós Salvador

机构信息

Institut Quimic de Sarria, Universitat Ramon Llull, Barcelona, Spain.

出版信息

Methods Mol Biol. 2013;1025:261-8. doi: 10.1007/978-1-62703-462-3_20.

DOI:10.1007/978-1-62703-462-3_20

PMID:23918344

Abstract

The surface modification of carbon nanotubes (CNTs) can be tailored to allow the formation of a complex between these potential carriers with DNA. In this chapter, protocols developed in our lab to prepare transfection vectors through the modification of MWCNTs are described. The protocol includes sections focused on the reduction of CNTs length, protocols to increase the dispersability of CNTs and finally, protocols for surface modification to attach through electrostatic interactions DNA to the CNTs.

摘要

碳纳米管（CNTs）的表面修饰可以进行定制，以使这些潜在载体与DNA之间形成复合物。在本章中，将描述我们实验室开发的通过修饰多壁碳纳米管（MWCNTs）来制备转染载体的方案。该方案包括专注于缩短碳纳米管长度的部分、提高碳纳米管分散性的方案，以及最终通过静电相互作用将DNA连接到碳纳米管上的表面修饰方案。