Division of Nanobiomedicine, and Division of Nano-Devices and Materials, Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences, Suzhou, P. R. China.
Bioconjug Chem. 2011 Nov 16;22(11):2237-43. doi: 10.1021/bc200189f. Epub 2011 Oct 25.
Functionalized multiwalled carbon nanotubes (f-MWNTs) are of great interest and designed as a novel gene delivery system. In this paper, we presented synthesis of polyamidoamine-functionalized multiwalled carbon nanotubes (PAA-g-MWNTs) and their application as a novel gene delivery system. The PAA-g-MWNTs, obtained from amide formation between PAA and chemically oxidized MWNTs, were stable in aqueous solution and much less toxic to cells than PAA and PEI 25KDa. More importantly, PAA-g-MWNTs showed comparable or even higher transfection efficiency than PAA and PEI at optimal w/w ratio. Intracellular trafficking of Cy3-labeled pGL-3 indicated that a large number of Cy3-labeled pGL-3 were attached to nucleus membrane, the majority of which was localized in nucleus after incubation with cells for 24 h. We have demonstrated that PAA modification of MWNTs facilitate higher DNA uptake and gene expression in vitro. All these facts suggest potential application of PAA-g-MWNTs as a novel gene vector with high transfection efficiency and low cytotoxicity.
功能化多壁碳纳米管（f-MWNTs）是非常有兴趣的，并设计为一种新型的基因传递系统。在本文中，我们介绍了聚酰胺-胺功能化多壁碳纳米管（PAA-g-MWNTs）的合成及其作为一种新型基因传递系统的应用。PAA-g-MWNTs 是通过 PAA 和化学氧化的 MWNTs 之间的酰胺形成获得的，在水溶液中稳定，比 PAA 和 PEI 25kDa 毒性小得多。更重要的是，在最佳 w/w 比下，PAA-g-MWNTs 显示出与 PAA 和 PEI 相当或更高的转染效率。Cy3 标记的 pGL-3 的细胞内运输表明，大量的 Cy3 标记的 pGL-3 附着在核膜上，其中大部分在与细胞孵育 24 小时后定位于核内。我们已经证明，MWNTs 的 PAA 修饰有助于提高体外 DNA 的摄取和基因表达。所有这些事实表明，PAA-g-MWNTs 作为一种具有高转染效率和低细胞毒性的新型基因载体具有潜在的应用前景。
