Gerats Tom, Zethof Jan, Vandenbussche Michiel
Department of Plant Genetics/IWWR/Radboud University, Nijmegen, The Netherlands.
Methods Mol Biol. 2013;1057:223-37. doi: 10.1007/978-1-62703-568-2_16.
Transposable genetic elements are considered to be ubiquitous. Despite this, their mutagenic capacity has been exploited in only a few species. The main plant species are maize, Antirrhinum, and Petunia. Representatives of all three major groups of class II elements, viz., hAT-, CACTA- and Mutator-like elements, have been identified in Petunia. Here we focus on the research "history" of the Petunia two-element Act1-dTph1 system and the development of its application in forward- and reverse-genetics studies.
转座遗传元件被认为是普遍存在的。尽管如此，它们的诱变能力仅在少数物种中得到利用。主要的植物物种是玉米、金鱼草和矮牵牛。在矮牵牛中已经鉴定出了II类元件的所有三个主要组的代表，即hAT、CACTA和Mutator样元件。在这里，我们重点关注矮牵牛双元件Act1-dTph1系统的研究“历史”及其在正向和反向遗传学研究中的应用发展。
