Southern California Coastal Water Research Project , Costa Mesa, California 92626, United States.
Environ Sci Technol. 2013 Oct 1;47(19):11268-77. doi: 10.1021/es401942n. Epub 2013 Sep 19.
Contaminants of emerging concern (CECs) in treated municipal effluents have the potential to adversely impact exposed organisms prompting elevated public concern. Using transcriptomic tools, we investigated changes in gene expression and cellular pathways in the liver of male fathead minnows (Pimephales promelas) exposed to 5% concentrations of full secondary-treated (HTP) or advanced primary-treated (PL) municipal wastewater effluents containing CECs. Gene expression changes were associated with apical end points (plasma vitellogenin and changes in secondary sexual characteristics). Of 32 effluent CECs analyzed, 28 were detected including pharmaceuticals, personal care products, hormones, and industrial compounds. Exposure to both effluents produced significantly higher levels of plasma VTG and changes in secondary sexual characteristics (e.g., ovipositor development). Transcript patterns differed between effluents, with <10% agreement in the detected response (e.g., altered production of transcripts involved in xenobiotic detoxification, oxidative stress, and apoptosis were observed following exposure to both effluents). Exposure to PL effluent caused changes in transcription of genes involved in metabolic pathways (e.g., lipid transport and steroid metabolism). Exposure to HTP effluent affected transcripts involved in signaling pathways (e.g., focal adhesion assembly and extracellular matrix). The results suggest a potential association between some transcriptomic changes and physiological responses following effluent exposure. This study identified responses in pathways not previously implicated in exposure to complex chemical mixtures containing CECs, which are consistent with effluent exposure (e.g., oxidative stress) in addition to other pathway responses specific to the effluent type.
处理后的城市废水中的新兴关注污染物（CECs）有可能对暴露的生物产生不利影响，引起公众的高度关注。本研究使用转录组工具，研究了暴露于含有 CECs 的全二级处理（HTP）或高级一级处理（PL）城市废水 5%浓度下的雄性胖头鱼（Pimephales promelas）肝脏中基因表达和细胞途径的变化。基因表达的变化与顶端终点（血浆卵黄蛋白原和第二性特征的变化）有关。在所分析的 32 种废水 CEC 中，检测到 28 种包括药物、个人护理产品、激素和工业化合物。暴露于这两种废水都会导致血浆 VTG 水平显著升高，并导致第二性特征发生变化（例如，产卵器发育）。废水之间的转录模式存在差异，检测到的反应一致性<10%（例如，暴露于这两种废水后，观察到参与异生物解毒、氧化应激和细胞凋亡的转录本的产生发生改变）。PL 废水暴露导致代谢途径（例如，脂质转运和类固醇代谢）相关基因的转录发生变化。HTP 废水暴露影响信号通路（例如，焦点黏附组装和细胞外基质）相关的转录本。结果表明，废水暴露后某些转录组变化与生理反应之间可能存在关联。本研究确定了与暴露于含有 CECs 的复杂化学混合物相关的以前未涉及的途径的反应，这些反应与废水暴露（例如氧化应激）以及特定于废水类型的其他途径反应一致。
