Hislop A A, Odom N J, McGregor C G, Haworth S G
Department of Paediatric Cardiology, Institute of Child Health, London, England.
J Thorac Cardiovasc Surg. 1990 Sep;100(3):360-70.
Syngeneic (Lewis to Lewis) and allogeneic (Brown Norway to Lewis) unilateral left lung transplants were performed on immature rats at 6 weeks of age at a time when alveoli are still multiplying after birth. Left lung denervation without transplantation was performed in a further group of rats (Lewis) by stripping the hilum, at 4 and 6 weeks of age. Animals were killed at either 2 weeks or 6 months after operation. Right and left lungs were analyzed separately by light microscopic quantitative techniques and findings were compared with findings from control animals matched for age and strain. The transplanted left lung in both syngeneic and allogeneic animals continued to grow to a normal size by formation of new alveoli, despite the presence of low-grade rejection activity in the immunosuppressed allogeneic group. The airways showed an increase in diameter for age at the hilum and periphery (p less than 0.01 and less than 0.001, respectively). The volume of the contralateral right lung was greater than normal because of an increase in number (p less than 0.01) and size of alveoli for age. Denervation alone was associated with normal growth of both lungs. Thus it appears that, in rats, the transplanted immature lung can fulfill its growth potential.
在出生后肺泡仍在增殖的6周龄未成熟大鼠身上进行了同基因（从Lewis大鼠到Lewis大鼠）和异基因（从棕色挪威大鼠到Lewis大鼠）的单侧左肺移植。在另一组大鼠（Lewis大鼠）中，于4周龄和6周龄时通过剥离肺门进行未移植的左肺去神经支配。在术后2周或6个月处死动物。通过光学显微镜定量技术分别分析右肺和左肺，并将结果与年龄和品系匹配的对照动物的结果进行比较。尽管免疫抑制的异基因组存在低度排斥反应，但同基因和异基因动物移植的左肺均通过形成新的肺泡继续生长至正常大小。气道在肺门和周边的直径随年龄增加（分别为p<0.01和p<0.001）。由于肺泡数量（p<0.01）增加和肺泡大小随年龄增大，对侧右肺体积大于正常。单纯去神经支配与双肺的正常生长相关。因此，在大鼠中，移植的未成熟肺似乎能够发挥其生长潜力。
