Minami M, Nakahara K, Matsumura A, Mizuta T, Yoon H E, Sakaguchi M, Mochizuki T, Yamatodani A, Matsuda H
First Department of Surgery, Osaka University Medical School, Suita, Japan.
J Heart Lung Transplant. 1995 May-Jun;14(3):505-11.
On the assumption that lung mast cells might respond and release histamine after lung transplantation, the number of mast cells and tissue histamine content were investigated for 2 weeks after rat orthotopic left lung isotransplantation (Lewis rat to Lewis rat) and allotransplantation (Lewis rat to Brown Norway rat). The allografts were rejected histologically by day 7 (grade A4).
In the isografts, both the numbers of mast cells and histamine content were lower (p < 0.05) on day 4 but higher (p < 0.001) on day 14 compared with the nontransplanted donor left lungs (control). In the allografts, the numbers of mast cells on day 7 and histamine content on day 1 were lower (p < 0.05) than controls and both continued to be low thereafter. On day 7, the histamine content of the allografts was low (p < 0.05) compared with the level of the isografts. Conversely, no significant change was found in histamine content of the untransplanted right native lungs except for on day 14 after isotransplantation, when the content was higher than the controls, suggesting the possibility of a systemic signal for proliferation of mast cells.
Our data indicated that degranulation of the pulmonary mast cells and consequent histamine release localized in the grafts occurred under the microenvironment of reimplantation and rejection after rat lung transplantation.
基于肺移植后肺肥大细胞可能做出反应并释放组胺的假设，对大鼠原位左肺同基因移植（Lewis大鼠至Lewis大鼠）和异基因移植（Lewis大鼠至Brown Norway大鼠）后2周内的肥大细胞数量和组织组胺含量进行了研究。同种异体移植物在第7天出现组织学排斥反应（A4级）。
与未移植的供体左肺（对照）相比，同基因移植物中肥大细胞数量和组胺含量在第4天较低（p<0.05），但在第14天较高（p<0.001）。在同种异体移植物中，第7天的肥大细胞数量和第1天的组胺含量低于对照，且此后两者均持续较低。在第7天，同种异体移植物的组胺含量与同基因移植物水平相比很低（p<0.05）。相反，未移植的右肺组织中组胺含量除在同基因移植后第14天高于对照外，未发现明显变化，提示可能存在肥大细胞增殖的全身信号。
我们的数据表明，大鼠肺移植后，在再植入和排斥反应的微环境下，肺肥大细胞脱颗粒并随之在移植物中释放组胺。
